Hot start Taq is a thermostable DNA polymerase that possesses a5′→3′ polymerase activity and a 5′ flap endonuclease activity. Hot Start Taq DNA Polymerase is a chemically modified enzyme that remains inactive at room temperature and requires an increase in temperature for activation. Hot start PCR reducesnon-specific amplification during setup stages of the reaction and helpsincrease PCR specificity and sensitivity. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to84%.
Product Includes
- Hot Start Taq DNA Polymerase
- 10x PCR Buffer with Mg2+
- 5x GC Enhancer
Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)
Concentration: 5 units/μlOne unit is defined as the amount of enzyme that incorporates 10 nmolesof dNTP into acid-insoluble form in 30 minutes at 72oC.
Source: E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus YT-1
Storage/Handling: Store at -20°C. Product may be shipped on blue ice.
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