Synonym:
Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)
Properties:
Related Categories |
Analytical/Chromatography,
Gel Filtration Chromatography,
Gel Filtration Media,
Molecular Biology,
Protein Chromatography,
|
form
|
suspension (20% aqueous ethanol)
|
feature
|
autoclavable
|
|
stability stable to 0.2M NaOH
|
matrix
|
crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer
|
particle size
|
25-75 μm (wet)
|
pore size
|
40-20,000 kDa MW range (dextrans)
|
compatibility
|
mode of use size exclusion chromatography
|
storage temp.
|
2-8°C
|
Description:
Application
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans.
Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.
General description
• Supplied swollen, suspended in 20% aqueous ethanol
• Autoclavable
• Stable to 0.2N sodium hydroxide (NaOH)
• Store at refrigerated temperature (2-8 °C)
Packaging
100, 250, 750 mL in glass bottle
Legal Information
Sephacryl is a registered trademark of GE Healthcare
Safety:
Symbol
GHS02
Personal Protective Equipment
Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
Flash Point(F)
100.4 - 109.4 °F
Flash Point(C)
38 - 43 °C