Rabbit polyclonal antibody raised against synthetic phosphopeptide of 14-3-3 protein.
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding S58 of rat 14-3-3 protein.
Host:
Rabbit
Theoretical MW (kDa):
29
Reactivity:
Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Primates, Rat, Sheep, Zebra fish
Form:
Liquid
Purification:
Affinity purification
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Recommend Usage:
Western Blot (1:1000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Datasheet:
Download
Publication Reference
1.
14-3-3 proteins: a number of functions for a numbered protein.Bridges D, Moorhead GB.Sci STKE. 2005 Aug 9;2005(296):re10.
2.
14-3-3 protein C-terminal stretch occupies ligand binding groove and is displaced by phosphopeptide binding.Silhan J, Obsilova V, Vecer J, Herman P, Sulc M, Teisinger J, Obsil T.J Biol Chem. 2004 Nov 19;279(47):49113-9. Epub 2004 Sep 4.
3.
Unlocking the code of 14-3-3.Dougherty MK, Morrison DK.J Cell Sci. 2004 Apr 15;117(Pt 10):1875-84.
Applications
Western Blot (Tissue lysate)
Western blot of rat brainstem lysate showing specific immuno- labeling of the ~29k 14-3-3 protein phosphorylated at Ser58 (Control, lane 1). The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos-pep, lane 2) but not by the corresponding dephosphopeptide (not shown).
Immunofluorescence
Immunostaining of mouse hippocampal cryosections with 14-3-3 (phospho S58) polyclonal antibody (Cat # PAB9657) (green). GFAP positive astrocytes (red) and DAPI labeled nuclei (blue). Photo courtesty of Robert Wine.