Chicken polyclonal antibody raised against human IgG (H&L).
Immunogen:
Human IgG whole molecule.
Host:
Chicken
Reactivity:
Human
Specificity:
IgG (H&L). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-chicken serum, human IgG and human serum.
Form:
Liquid
Conjugation:
Unconjugated
Purification:
This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Isotype:
IgG
Recommend Usage:
ELISA (1:20000-1:60000)Immunohistochemistry (1:1000-1:5000)Western Blot (1:2000-1:10000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (0.01% (w/v) sodium azide).
Storage Instruction:
Store at 4°C. For long term storage, aliquot contents and freeze at -20°C or below.Aliquot to avoid repeated freezing and thawing.This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
ELISA results of this antibody tested against purified human IgG. Each well was coated in duplicate with 10 ug of human IgG. The working dilution of Anti-Human IgG is 1:110,000. The starting dilution of antibody was 5 ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Chicken IgG mx10 HRP conjugated and TMB substrate.
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