Goat polyclonal antibody raised against native LDHA (Rabbit).
Immunogen:
Native purified LDHA from rabbit muscle.
Host:
Goat
Reactivity:
Rabbit
Form:
Lyophilized
Isotype:
IgG
Quality Control Testing:
Antibody Reactive Against Native Purified Protein.
Recommend Usage:
ELISA (1:2000 - 1:10000)Immunoprecipitation (1:100)Western Blot (1:1000-1:5000)The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction:
Store at 4°C on dry atmosphere.After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
Download
Applications
Western Blot (Cell lysate)
Western Blot analysis of HeLa cell extracts by LDHA polyclonal antibody (Cat # PAB27911) with 30 ug of protein at 1:400. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Detected LDH at 36.6 kDa and 36 kDa .