Enzyme Commission (EC) Number 3.4.21.19 ( BRENDA | IUBMB )
MDL number MFCD01324998
Popular Documents: Bulletin (PDF) | Specification Sheet (PDF)
Properties:
Related Categories
3.4.x.x Peptidases,
3.x.x.x Hydrolases,
Analytical Reagents,
Analytical/Chromatography,
Application Specific Reagents,
Biochemicals and Reagents,
Endoproteinase Glu-C,
Enzymatic and Chemical Protein Cleavage,
Enzyme Class Index,
Enzymes, Inhibitors, and Substrates,
Mass Spectrometry,
Molecular Biology,
Plant Biotechnology,
Plant Proteomics,
Products for Amino Acid Hydrolysis,
Proteases for Cleavage of Proteins,
Protein Digest,
Protein Identification,
Proteolytic Enzymes,
Proteolytic Enzymes and Substrates,
Proteomics,
Reagents for Amino Acid Analysis,
Selective Proteolytic Enzymes
More...
form
lyophilized powder
suitability
suitable for protein sequencing
storage temp.
−20°C
Description:
Application
• Endoproteinase Glu-C from Staphylococcus aureus V8 has been used for obtaining proteolytic cleavage fragments of the S-layer protein (from Bacillus stearothermophilus ATCC 12980) to perform affinity studies. • It has been used for the enzymatic cleavage of native VSTx-3 (voltage sensor toxin 3) peptide for its sequence determination. • It has been used for limited proteolysis of recombinant purified PimA (phosphatidylinositol mannosyltransferase). • It has been used for the digestion of glycosylated hemoglobin for isotope dilution liquid chromatography-tandem mass spectrometry analysis.
Biochem/physiol Actions
Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine endoprotease, which hydrolyzes peptide bonds at the carboxyl side of glutamyl and aspartyl residues. The specificity of Glu-C is dependent upon the buffer and pH employed as well as the structure around the potential cleavage site. In ammonium acetate (pH 4.0) or ammonium bicarbonate (pH 7.8), the enzyme preferentially cleaves glutamyl bonds; whereas, in phosphate buffer (pH 7.8) Glu-C will cleave at either site. Glu-C is reported to be active in the presence of 0.2% SDS (sodium dodecyl sulfate) and in 4.0M urea.
Safety:
Symbol
GHS08
Signal word
Danger
Hazard statements
H351-H360
Precautionary statements
P201-P280-P308 + P313
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Faceshields, Gloves