The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
WEE1 (Phospho-Ser642) Antibody
Synonyms
WEE1hu; Wee1-like protein kinase; kinase WEE1
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human WEE1 around the phosphorylation site of Ser642.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
WEE1 (Phospho-Ser642) Antibody detects endogenous levels of WEE1 only when phosphorylated at Ser642.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
WEE1
Database Links
Gene ID: 7465 Swiss-Prot #: P30291
OMIM #: 193525 Unigene #: Hs.249441
Target Modification
Phospho
Modification Sites
H:S642, M:S642, R:S642
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using WEE1 (Phospho-Ser642) Antibody
Image 2
Western blot analysis of lysates from 293 cells treated with etoposide 25uM 60', using WEE1 (Phospho-Ser642) Antibody.