The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
RFA2 (Phospho-Ser33) Antibody
Synonyms
REPA2; replication factor-A protein 2; replication protein A 32 kDa subunit; replication protein A2; RF-A; RP-A; RPA2; RPA32
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human RFA2 around the phosphorylation site of Ser33.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
RFA2 (Phospho-Ser33) Antibody detects endogenous levels of RFA2 only when phosphorylated at Ser33.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using RFA2 (Phospho-Ser33) Antibody
Image 2
Western blot analysis of lysates from NIH/3T3 cells treated with Adriamycin 0.5ug/ml 24h, using RFA2 (Phospho-Ser33) Antibody.