The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Retinoblastoma (Phospho-Ser608) Antibody
Synonyms
P105-RB; PP105; PP110; RB-1; RB1; retinoblastoma gene; Retinoblastoma-associated protein
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human Retinoblastoma around the phosphorylation site of Ser608.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
Retinoblastoma (Phospho-Ser608) Antibody detects endogenous levels of RB1 only when phosphorylated at Ser608.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RB1
Database Links
Gene ID: 5925 Swiss-Prot #: P06400
OMIM #: 109800/180200/259500 Unigene #: Hs.408528
Target Modification
Phospho
Modification Sites
H:S608, M:S601, R:S579
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Retinoblastoma (Phospho-Ser608) Antibody
Image 2
Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30', using Retinoblastoma (Phospho-Ser608) Antibody.