The antiserum was produced against synthesized phospho-peptide derived from human PLK1 around the phosphorylation site of Ser137.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
PLK1 (Phospho-Ser137) Antibody detects endogenous levels of PLK1 only when phosphorylated at Ser137.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PLK1
Database Links
Gene ID: 5347 Swiss-Prot #: P53350
OMIM #: 602098 Unigene #: Hs.592049
Target Modification
Phospho
Modification Sites
H:S137, M:S137, R:S137
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLK1 (Phospho-Ser137) Antibody
Image 2
Western blot analysis of lysates from Jurkat cells treated with PMA, using PLK1 (Phospho-Ser137) Antibody.