The antiserum was produced against synthesized phospho-peptide derived from human Mnk1 around the phosphorylation site of Thr385.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
Mnk1 (Phospho-Thr385) Antibody detects endogenous levels of MKNK1 only when phosphorylated at Thr385.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MKNK1
Database Links
Gene ID: 8569 Swiss-Prot #: Q9BUB5
OMIM #: 606724 Unigene #: Hs.371594
Target Modification
Phospho
Modification Sites
H:T385, M:T344, R:T332
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Mnk1 (Phospho-Thr385) Antibody
Image 2
Western blot analysis of lysates from COLO205 cells treated with PMA 125ng/ml 30', using Mnk1 (Phospho-Thr385) Antibody.