The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
IRF-3 (Phospho-Ser396) Antibody
Synonyms
Interferon regulatory factor 3; IRF3
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human IRF-3 around the phosphorylation site of Ser396.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
IRF-3 (Phospho-Ser396) Antibody detects endogenous levels of IRF3 only when phosphorylated at Ser396.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
IRF3
Database Links
Gene ID: 3661 Swiss-Prot #: Q14653
OMIM #: 603734 Unigene #: Hs.75254
Target Modification
Phospho
Modification Sites
H:S396, M:S388, R:S390
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRF-3 (Phospho-Ser396) Antibody
Image 2
Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using IRF-3 (Phospho-Ser396) Antibody.