The antiserum was produced against synthesized phospho-peptide derived from human Integrin beta1 around the phosphorylation site of Thr789.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
Integrin beta1 (Phospho-Thr789) Antibody detects endogenous levels of ITGB1 only when phosphorylated at Thr789.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ITGB1
Database Links
Gene ID: 3688 Swiss-Prot #: P05556
OMIM #: 135630 Unigene #: Hs.713531
Target Modification
Phospho
Modification Sites
H:T789, M:T789, R:T790
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Integrin β1 (Phospho-Thr789) Antibody
Image 2
Western blot analysis of lysates from HepG2 cells treated with Ca2+ 40uM 30', using Integrin beta1 (Phospho-Thr789) Antibody.