The antiserum was produced against synthesized phospho-peptide derived from human ICK around the phosphorylation site of Tyr159.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
ICK (Phospho-Tyr159) Antibody detects endogenous levels of ICK only when phosphorylated at Tyr159.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ICK
Database Links
Gene ID: 22858 Swiss-Prot #: Q9UPZ9
OMIM #: 612325 Unigene #: Hs.417022
Target Modification
Phospho
Modification Sites
H:Y159, M:Y159, R:Y159
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ICK (Phospho-Tyr159) Antibody
Image 2
Western blot analysis of lysates from NIH/3T3 cells treated with starved 24h, using ICK (Phospho-Tyr159) Antibody.