The antiserum was produced against synthesized phospho-peptide derived from human eNOS around the phosphorylation site of Ser615.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
eNOS (Phospho-Ser615) Antibody detects endogenous levels of NOS3 only when phosphorylated at Ser615.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NOS3
Database Links
Gene ID: 4846 Swiss-Prot #: P29474
OMIM #: 163729 Unigene #: Hs.647092
Target Modification
Phospho
Modification Sites
H:S615, M:S614, R:S614
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eNOS (Phospho-Ser615) Antibody
Image 2
Western blot analysis of lysates from K562 cells treated with EGF 40nM 30', using eNOS (Phospho-Ser615) Antibody.