The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Vimentin (Phospho-Ser72) Antibody
Synonyms
None
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human Vimentin around the phosphorylation site of Ser72.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
Vimentin (Phospho-Ser72) antibody detects endogenous levels of Vimentin only when phosphorylated at Ser72.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
VIM
Database Links
Gene ID: 7431 Swiss-Prot #: P08670
OMIM #: 193060 Unigene #: Hs.642813
Target Modification
Phospho
Modification Sites
H:S72, M:S72, R:S72
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Vimentin (Phospho-Ser72) Antibody
Image 2
Western blot analysis of lysates from 293 cells treated with paclitaxel, using p-Vimentin (Phospho-Ser72) antibody.