The antiserum was produced against synthesized phospho-peptide derived from human Na+/K+-ATPase alpha1 around the phosphorylation site of Tyr260.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
Na+/K+-ATPase alpha1 (Phospho-Tyr260) antibody detects endogenous levels of Na+/K+-ATPase alpha1 only when phosphorylated at Tyr260.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ATP1A1
Database Links
Gene ID: 476 Swiss-Prot #: P05023
OMIM #: 182310 Unigene #: Hs.371889
Target Modification
Phospho
Modification Sites
H:Y260, M:Y260, R:Y260
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Na+/K+-ATPase α1 (Phospho-Tyr260) Antibody
Image 2
Western blot analysis of lysates from 293 cells treated with PMA, using phospho-Na+/K+-ATPase α1 (Phospho-Tyr260) antibody.