The antiserum was produced against synthesized phospho-peptide derived from human LKB1 around the phosphorylation site of Ser428.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
LKB1 (Phospho-Ser428) antibody detects endogenous levels of LKB1 only when phosphorylated at Ser428.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
STK11
Database Links
Gene ID: 6794 Swiss-Prot #: Q15831
OMIM #: 175200/273300/602216 Unigene #: Hs.515005
Target Modification
Phospho
Modification Sites
H:S428, M:S428
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using LKB1 (Phospho-Ser428) Antibody
Image 2
Western blot analysis of lysates from forsklin treated CV-1 cells, using phospho-LKB1 (Phospho-Ser428) antibody.
Image 3
Immunohistochemistry analysis of phospho-LKB1 (Phospho-Ser428) antibody in paraffin-embedded human breast carcinoma tissue.