The antiserum was produced against synthesized phospho-peptide derived from human Fyn around the phosphorylation site of Tyr213.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
Fyn (Phospho-Tyr213) antibody detects endogenous levels of Fyn only when phosphorylated at Tyr213.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
FYN
Database Links
Gene ID: 2534 Swiss-Prot #: P06241
OMIM #: 137025 Unigene #: Hs.390567
Target Modification
Phospho
Modification Sites
H:Y213, M:Y213, R:Y213
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Fyn (Phospho-Tyr213) Antibody
Image 2
Western blot analysis of lysates from brain tissue, using phospho-Fyn (Phospho-Tyr213) antibody.