The antiserum was produced against synthesized phospho-peptide derived from human EphA2 around the phosphorylation site of Tyr772.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
EphA2 (Phospho-Tyr772) antibody detects endogenous levels of EphA2 only when phosphorylated at Tyr772.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
EPHA2
Database Links
Gene ID: 1969 Swiss-Prot #: P29317
OMIM #: 176946 Unigene #: Hs.171596
Target Modification
Phospho
Modification Sites
H:Y772, M:Y773
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using EphA2 (Phospho-Tyr772) Antibody
Image 2
Western blot analysis of lysates from brain tissue, using phospho-EphA2 (Phospho-Tyr772) antibody.