The antiserum was produced against synthesized phospho-peptide derived from human ATP-citrate synthase around the phosphorylation site of Ser455.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
ATP-citrate synthase (Phospho-Ser455) antibody detects endogenous levels of ATcitrate synthase only when phosphorylated at Ser455.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ACLY
Database Links
Gene ID: 47 Swiss-Prot #: P53396
OMIM #: 108728 Unigene #: Hs.387567
Target Modification
Phospho
Modification Sites
H:T447+S451, M:T447+S451, R:T446+S450
Application Images
Image 2
Western blot analysis of lysates from COS7 cells treated with Calyculin, using phospho-ATP-citrate synthase (Phospho-Ser455) antibody.