The antiserum was produced against synthesized phospho-peptide derived from human ACK around the phosphorylation site of Tyr518.
Immunogen Range
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
ACK (Phospho-Tyr518) antibody detects endogenous levels of ACK only when phosphorylated at Tyr518.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
TNK2
Database Links
Gene ID: 10188 Swiss-Prot #: Q07912
OMIM #: 606994 Unigene #: Hs.518513
Target Modification
Phospho
Modification Sites
H:Y518, R:Y518
Application Images
Image 1
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ACK (Phospho-Tyr518) Antibody
Image 2
Western blot analysis of lysates from HepG2 cells treated with EGF, using phospho-ACK (Phospho-Tyr518) antibody.