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NEB/pSNAPf Vector/20 μg/N9183S
  • NEB/pSNAPf Vector/20 μg/N9183S

NEB/pSNAPf Vector/20 μg/N9183S

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貨號(hào): N9183S
品牌: NEB
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  • 使用說(shuō)明
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    • pSNAPf Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of SNAP-tag® protein fusions in mammalian cells. This plasmid encodes SNAPf, a SNAP-tag protein, which is expressed under control of the CMV promoter. The expression vector has an IRES (internal ribosome entry site) and a neomycin resistance gene downstream of the SNAPf for the efficient selection of stable transfectants. pSNAPf Vector contains two multiple cloning sites to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the SNAPf.The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzyl purines and benzyl pyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.pSNAPf contains an improved version of SNAP-tag, termed SNAPf. SNAPf displays faster kinetics in in vitro labeling and fast, specific and efficient labeling in live and fixed cell applications, thereby rendering it a desired research tool for analysis of protein dynamics.

      There are two steps to using this system: sub cloning and expression of the protein of interest as a SNAPf fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Cloning and expression of SNAPf fusion proteins are described in this document. The labeling of the fusion proteins with SNAP-tag substrates is described in the instructions supplied with the SNAP-tag substrates.

      Cloning Region ofpSNAPCloning Region of pSNAP Unique restriction sites in theregions flanking the SNAPf gene are displayed above thecoding strand. The complete sequence for pSNAPf and the controlplasmids can be downloaded.
      This product is related to the following categories:
      Cellular Analysis Vectors Products,
      DNA Plasmids & Substrates Products,
      Protein Labeling Products
      This product can be used in the following applications:
      Snap Surface Products,
      In vivo Imaging,
      Pulse Chase,
      Receptor Internalization,
      Protein Localization,
      Protein Labeling Snap Clip
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