Description:
IMPACTCitations
pTXB1isanE.coliexpressionvectorintheIMPACT?Kit(NEB#E6901)(1,2).Itisdesignedforthein-frameinsertionofatargetgeneintothepolylinkerupstreamoftheMxeintein/chitinbindingdomain(27kDa)(2,3,4,5).Thefusionproteinisboundtochitinbeadsandthethiol-inducedcleavageactivityoftheinteinreleasesthetargetprotein.pTXBvectorsarerecommendedforuseinintein-mediatedproteinligationandC-terminallabeling(2).Thisdoublestrandedvectoris6,706basepairsinlength.
DNASUandAddgenearecentralrepositoriesforplasmidclonesandcollectionsthatmayalsobehelpful.