Synonym:AcAcAssayKitProperties:
RelatedCategories | AssayKitsForStudyingFattyAcidMetabolism,BloodandUrineChemistryAssayKits,CancerMetabolism,CancerResearch,CellBIOLOGy, |
storagetemp. | −20°C |
Description:
Generaldescription
Acetoacetate(AcAc)isaβ-ketoacidformedinthemitochondriabythecondensationoftwomoleculesofacetyl-CoA.AcAciseitherenzymaticallyreducedto3-hydroxybutyrateordecarboxylatedtoproduceacetone.Ketonebodies(AcAc,3-HB,andacetone)areusedasalternatesourceofenergyintheabsenceofglucose.ExcessketonebodiesareobservedintypeIdiabetes,alcoholism,orstarvation.
TheAcetoacetateColorimetricAssaykitprovidesasimpleandsensitiveprocedureformeasuringAcAcinvarioussamples(rangingfrom20-100nmole/well).AcAcconcentrationisdeterminedbygeneratingacolorimetric(550nm)productproportionaltotheamountofAcAcpresent.Thekitdoesnotdetect3-hydroxybutyrateandisspecificforacetoacetate.
SuitABIlity
Suitableforthedetectionofacetoacetateinsamplessuchasserum,plasma,blood,urine,andotherbodyfluids.
Safety:
Symbol
GHS07
Hazardstatements
H315-H319-H335
Precautionarystatements
P261-P305+P351+P338
RIDADR
NONHforallmodesoftransport