Properties:
RelatedCategories | AminoAcidMetabolismAssayKits,BloodandUrineChemistryAssayKits,CellBIOLOGy,EnzymaticActivityAssayKits,EnzymaticKitsfortheQuantitationofNutrientsandMetabolites, |
usage | sufficientfor200 colorimetric?tests |
GeneInformation | human...ARG1(383),ARG2(384) mouse...ARG1(11846),ARG2(11847) rat...ARG2(29215),Arg1(29221) |
Description:
FeaturesandBenefits
CompatIBLewithhigh-throughputhandlingsystems.
OtherNotes
Thiskitisshippedatroomtemperature.StoretheArginaseBufferandUreastandardat−20°C.Allothercomponentsarestoredat2-8°C.
Generaldescription
Arginaseisamanganese-containingenzymethatcatalyzestheconversionofargininetoureaandornithine.Twoisoformsofarginasearepresentinmostmammalswhichdifferintheirtissuedistributionandsubcellularlocalization.ArginaseIisacytoplasmicproteinpredominantlyexpressedintheliverwhereitcatalyzesthefifthandfinalstepoftheUreaCycle.ArginaseIIisamitochondrialproteinwithbroadertissuedistributionwhosefunctionmayincludenitricoxideandpolyaminemetabolism.DecreasedactivityorexpressionofArginaseIresultsintheautosomalrecessivedisorderhyperarginemia.IncreasedserumArginaseactivityisimplicatedinhepaticinjuryandincertainpathologicalconditionssuchascancer.Arginasealsoparticipatesinmultipleinflammatoryreactionsintheimmunesystem.
Inthisassay,arginasecatalyzestheconversionofargininetoureaandornithine.Theureaproducedspecificallyreactswiththesubstratetogenerateacoloredproduct,proportionaltothearginaseactivitypresent.
SuitABIlity
Suitableforthedetectionofarginaseactivityinenzymepreparations,serum,plasma,tissueculture,andsimilarsamples.Suitableforstudyingtheeffectsofdrugsonarginaseactivity.
Safety:
Hazardstatements
H302-H314-H317-H360FD-H400
Precautionarystatements
P201-P273-P280-P305+P351+P338-P310