Properties:
RelatedCategories | AminoAcidMetabolismAssayKits,BloodandUrineChemistryAssayKits,CellBIOLOGy,EnzymaticActivityAssayKits,EnzymaticKitsfortheQuantitationofNutrientsandMetabolites, |
usage | sufficientfor100 colorimetric?tests |
storagetemp. | −20°C |
GeneInformation | human...GLUD1(2746),GLUD2(2747) mouse...GLUD1(14661) rat...GLUD1(24399) |
Description:
FrequentlyAskedQuestions
FrequentlyAskedQuestionsareavailableforthisProduct.
Generaldescription
GlutamateDehydrogenase(GDH)isamitochondrialenzymethatcatalyzesthereversIBLeoxidativedeaminationofglutamatetoα-ketoglutarateandservesasakeylinkbetweenanabolicandcatabolicpathways.Inmammals,GDHissubjecttoallostericregulationandhashighactivityinliver,kidney,brain,andpancreas.GDHactivityinserumcanbeusedtodifferentiatebetweenliverdiseasesduetoliverinflammation,whichdonotshowelevatedserumGDHactivity,anddiseasesthatresultinhepatocytenecrosis,whichresultsinelevatedserumGDH.
GDHactivityisdeterminedbyacoupledenzymeassayinwhichglutamateisconsumedbyGDHgeneratingNADH,whichreactswithaprobegeneratingacolorimetric(450nm)productproportionaltotheGDHactivitypresent.OneunitofGDHistheamountofenzymethatwillgenerate1.0μmoleofNADHperminuteatpH7.6at37°C.
Safety:
RIDADR
NONHforallmodesoftransport