當前位置 : Millipore >>> Millipore/MAB5304 | Anti-Glutamate Antibody/MAB5304/100 µL
Millipore/MAB5304 | Anti-Glutamate Antibody/MAB5304/100 µL
  • Millipore/MAB5304 | Anti-Glutamate Antibody/MAB5304/100 µL

Millipore/MAB5304 | Anti-Glutamate Antibody/MAB5304/100 µL

價格: ¥4308.00 市場價: 7180.00

貨號: MAB5304
品牌: Millipore
規(guī)格
數(shù)量
庫存(0)
特別 提示
代購產品:無質量問題不接受退換貨,下單前請仔細核對信息。
下單后請及時聯(lián)系客服核對商品價格,訂單生效后再付款。
資深產品顧問
咨詢顧問

全國免費服務熱線

4000-520-616


  • 自營商城 一站式服務
  • 廠家直采 剔除溢價
  • 品質甄選 正品保證
  • 嚴控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說明
  • 常見問題
    • Description
      CatalogueNumberMAB5304
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-GlutamateAntibody
      ProductInformation
      FormatPurified
      PresentationLiquidinPBScontaining10mMsodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainat2-8°Cinundilutedaliquotsforupto6monthsafterdate.
      Applications
      ApplicationAnti-GlutamateAntibodydetectslevelofGlutamate&hasbeenpublished&validatedforuseinIH.
      KeyApplications
      • Immunohistochemistry
      ApplicationNotesImmunohistochemistry:1:2,500-1:10,000usingfreefloatingsectionsbythePAPtechniqueonrathippocampus.

      Optimalworkingdilutionsmustbedeterminedbyenduser.

      PROTOCOLforGlutamateDetectionbyImmunohisto/cytochemistry.Exampleforaratbrain.

      1.SOLUTIONSTOBEPREPARED-Solutionmustbepreparedasneeded.

      SolutionA:0.1Mcacodylate,10g/LsodiummetABIsulfite,pH6.2.

      SolutionB:0.1Mcacodylate,10g/Lsodiummetabisulfite,3-5%glutaraldehyde,pH7.5.

      2.RATPERFUSION-TheratisanaesthetizedwithsodiumpentobarbitalorNembutalandperfusedintracardiallythroughtheaortausingapumpwithSolutionA(30mL):150-300mL/min,SolutionB(500mL):150-300mL/min.

      3.POSTFIXATION:15to30minutesinSolutionB,then4softwashesin0.05MTriswith8.5g/Lsodiummetabisulfite,pH7.5(SolutionC).

      4.TISSUESECTIONING:Vibratomeorcryostatsectionscanbeused.

      5.REDUCTIONSTEP:SectionsarereducedwithSolutionCcontaining0.1Msodiumborohydridefor10minutes.Thesectionsarewashed4timesinsolutionCwithoutsodiumborohydride.

      6.APPLICATIONOFGLUTAMATEANTIBODY:Useafinaldilutionof1:2,500-1:10,000inSolutionCcontaining0.1%TritonX100and2%non-specificserum.Incubate12sectionsper2mLdilutedantibodyovernight,+2-8°C.Thenwashthesectionsthreetimesfor10minuteseachinSolutionC.(Notethattheantibodymaybeusableatahigherdilution.Thisshouldbeexploredtominimizethepossibilityofhighbackground.Additionally,notethatachangeinthebufferingsystemasindicatedintheprotocolmaychangethebackgroundandantibodyrecognition).ThespecificreactionisthenrevealedbyPAPprocedure.

      7.SECONDANTIBODY:Incubatethesectionswitha1:50to1:200dilutionofgoatanti-mouseinSolutionBcontaining1%non-specificserumforeither3hrsat20°Cor1-2hrat37°C.Thenwashthesections,3times,for10minuteseachwithSolutionC.

      8.PAP:Incubatethesectionswiththeappropriatedilutionofperoxidaseanti-peroxidase(forfreefloatingmethod)inSolutionCfor1-2hoursat37°C.Thenwashsections3timesfor10mineachinsolutionC.

      9.VISUALIZATION:Theantigen-antibodycomplexesarevisualizedusingDAB-4-HCl(25mg/100mL)(orotherchromogen)in0.05MTrisandfiltrated;0.05%hydrogenperoxideisadded.Incubatethesectionsfor10minutesatroomtemp.Stopthereactionbytransferringthesectionsto5mL0.05MTris.Mountsectionsonchrome-alumcoatedslides.Dryovernightat37°C.Rehydratesectionsusingconventionalhistologicalprocedures.Coverslipusingrapidmountingmedia.

      Forresearchuseonly;notforuseasadiagnostic.
      BIOLOGicalInformation
      ImmunogenGlutamate-Glutaraldehyde-BSA.
      HostMouse
      SpecificityGlutamate

      Thecross-reactivitiesweredeterminedusinganELISAtestbycompetitionexperimentswiththefollowingcompounds:

      CompoundCross-reactivity

      Glutamate-G-BSA1

      Aspartate-G-BSA1/100,000

      GABA-G-BSA1/100,000

      Glutamate1/100,000

      Abbreviations:

      (G)Glutaraldehyde

      (BSA)BovineSerumAlbumin

      NOTE:AntibodyreactivityREQUIRESglutaraldehydefixationthussomeglutaraldehyde(0.5%-2.0%)needstobeincludedinthetissuefixationprocedureinorderfortheproperreactivity.
      IsotypeIgM
      SpeciesReactivity
      • Rat
      AntibodyTypeMonoclonalAntibody
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    螞蟻淘生物188,秉承螞蟻淘一貫的嚴謹態(tài)度,由螞蟻淘公司專業(yè)人員負責品控、采購、物流、銷售、售后,保障正品優(yōu)質。以“快速好省,為科研提供好產品、好價格”為理念,直接鏈接原廠家,從全國各地原制造商嚴格挑選188款科研精品,剔除品牌溢價,188生物新電商,把好的產品帶給科研!? 力求給你最優(yōu)質的商品。
  • Q:生物188產品正品保障嗎?
    A:生物188質量把控人員具有十年的從業(yè)經驗,在業(yè)界享有良好的口碑;自營商城,直接從廠家采購, 自己的團隊負責國際物流和清關,中間沒有第三方,所有流程嚴格把控,100%保證正品,假一罰十。

    Q:下單后可以修改訂單嗎?
    A:下單后的商品付款之前可以修改;訂單付款成功,需要聯(lián)系我們客服進行修改;客服電話:4000-520-616

    Q:可以開發(fā)票嗎?
    A:本網站所售商品都是正規(guī)清關,均開具16%正規(guī)發(fā)票,發(fā)票金額含配送費金額,另有說明的除外。

    Q:商品幾天可以發(fā)貨?
    A:生物188商品,全部現(xiàn)貨銷售,付款后即可發(fā)貨,一般一周內送達!

    Q:如何聯(lián)系商家?
    A:有任何問題夠可以電話咨詢我們,全國24小時免費服務熱線:4000-520-616 或聯(lián)系我們的在線客服QQ:1570468124

    Q:收到的商品少了/發(fā)錯了怎么辦?
    A:同個訂單購買多個商品可能會分為一個以上包裹發(fā)出,可能不會同時送達,建議查看訂單詳情是否是 部分發(fā)貨狀態(tài);如未收到,可聯(lián)系在線客服或者致電4000-520-616。

    Q:退換貨/維修需要多長時間?
    A:一般情況下,退貨處理周期為客戶收到產品一個月內(以快遞公司顯示簽收時間為準),包裝規(guī)格、 數(shù)量、品種不符,外觀毀損、短缺或缺陷,請在收到貨24小時內申請退換貨;特殊商品以合同條款為準。

何為188

極簡而嚴謹,我們僅銷售188款生物醫(yī)學科研用品,款款都是爆款;因為少所以聚焦,聚焦甄選每一款產品,聚焦服務每一位客戶!

關注我們 :

點擊QQ聯(lián)系我們
生物188微信

關注188微信公眾號

獲取最新優(yōu)惠活動通知
  • 品質甄選,正品保證

  • 自營電商,廠家直采

  • 極簡主義,188精品