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Millipore/MAB5266 | Anti-Neurofilament 200 kDa Antibody, clone N52/MAB5266/50 µg
  • Millipore/MAB5266 | Anti-Neurofilament 200 kDa Antibody, clone N52/MAB5266/50 µg

Millipore/MAB5266 | Anti-Neurofilament 200 kDa Antibody, clone N52/MAB5266/50 µg

價(jià)格: ￥3576.00 市場(chǎng)價(jià): 5960.00
貨號(hào): MAB5266
品牌: Millipore
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    • Description
      CatalogueNumberMAB5266
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-Neurofilament200kDaAntibody,cloneN52
      BackgroundInformationNeurofilamentsareatypeofintermediatefilamentthatserveasmajorelementsoftheCytoskeletonsupportingtheaxoncytoplasm.Theyarethemostabundantfibrillarcomponentsoftheaxon,beingonaverage3-10timesmorefrequentthanaxonalmicrotubules.Neurofilaments(10nmindia.)arebuiltfromthreeintertwinedprotofibrilswhicharethemselvescomposedoftwotetramericprotofilamentcomplexsofmonomericproteins.Theneurofilamenttripletproteins(68/70,160,and200kDa)occurinboththecentralandperipheralnervoussystemandareusuallyneuronspecific.The68/70kDaNF-Lproteincanself-assembleintoafilamentousstructure,howeverthe160kDaNF-Mand200kDaNF-Hproteinsrequirethepresenceofthe68/70kDaNF-Lproteintoco-assemble.Neuromas,ganglioneuromas,gangliogliomas,ganglioneuroblastomasandneuroblastomasstainpositivelyforneurofilaments.Althoughtypicallyrestrictedtoneurons,neurofilamentshavebeendetectedinparagangliomasandadrenalandextra-adrenalpheochromocytomas.Carcinoids,neuroendocrinecarcinomasoftheskin,andoatcellcarcinomasofthelungalsoexpressneurofilaments.FormoreneurofilamentinformationseeNervousSystemCellTypeSpecificMarkerchartonlineundertheCHEMICONTechnicalSupportsection.
      ProductInformation
      FormatPurified
      PresentationPurifiedimmunoglobulin(Ionexchangechromatography).Liquidin0.02Mphosphatebuffer,0.25MNaClwith0.1%sodiumazide,pH7.6
      StorageandShippingInformation
      StorageConditionsMaintainat2-8°Cinundilutedaliquotsforupto6months.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationThisAnti-Neurofilament200kDaAntibody,cloneN52isvalidatedforuseinWB,IHforthedetectionofNeurofilament200kDa.
      KeyApplications
      • WesternBlotting
      • Immunohistochemistry
      ApplicationNotesWesternblot:5-10μg/mL

      Immunohistochemistry:5-10μg/mL

      Optimalworkingdilutionsmustbedeterminedbyenduser.

      Immunohistochemistry:AntibodyN52reactswithafragmentofNF-200side-armthatislocatedattheendoftheMPRKSPdomainandwhichcontainstheconsensuscdk-5phosphorylationsite,howeverthisreactivityisabolishediftheNF-200fragmentbecomesphosphorylatedbycdk-5{Guidatoetal,1996}.Thusforthefulleststainingandreactivity,includingwesterns,itissuggestedthatsamplesbetreatedwithalkalinephosphatasepriortoantibodystaining.Thefollowingtreatmentprotocolissuggested:



      ProtocolPreparationofSolutions:

      I.TBS:Tris-HCl,0.1mol/l;NaCl,0.1mol/l"pH8.0.

      II.Mixalkalinephosphatase,1mg/mlwith1mol/lZnSO4,;1mol/lMgCl2,and1mmol/lPMSF;anddissoveinTBS.

      NOTE:ItisnecessarytodialyzethealkalinephosphataseagainstsolutionIbeforeuse.

      Procedure:

      Frozensectionsfromshockfrozentissuesamplesareair-driedandthenfixedwithacetonefor10minat-20°C.Theexcessacetoneisallowedtoevaporateatroomtemperature.IncubatethesectionsinsolutionsIIfor4hoursat+30oCandwashinTBS3timesfor5minuteseach.NegativecontrolsectionsareincubatedinsolutionIIwithoutalkalinephosphatase.Furthertreatmentasfollows:

      -Coverthepreparationwith10-20μlantibodysolutionandincubatefor1hrinahumidchamber.

      -ImmersetheslideinTBSandwashinTBS3timesfor5minuteseach.

      -Coverthepreparationwith10-20μlofasolutionofanti-mouseIg-antibody,labeledwithfluorscineisothiocyanate,andincubateinahumidchamberat37°Cfor1hour.

      -WashtheslideinTBS3timesfor5mineach.
      BIOLOGicalInformation
      ImmunogenCarboxyterminaltailsegmentofenzymaticallydephosphorylatedporcineH-chain.
      CloneN52
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityMAB5266reactswiththephophorylatedanddephosphorylatedH-chainofneurofilament200kDa(NF-H)innormaltissues/extracts(Shaw,1986).MAB5266canbeusedtodetectcellsofneuronaloriginbyimmunohistochemistryandWesternblot.IthasbeenreportedthatreactivityofMAB5266withNF-Hisblockedincdk-5over-expressingcells(Guidato,1996).
      IsotypeIgG1
      SpeciesReactivity
      • Human
      • Mouse
      • Monkey
      • Pig
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • NEFH
      • NFH
      • NF-H
      • KIAA0845
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P12036#Neurofilamentsusuallycontainthreeintermediatefilamentproteins:L,M,andHwhichareinvolvedinthemaintenanceofneuronalcaliber.NF-HhasanimportantfunctioninmatureaxonsthatisnotsubservedbythetwosmallerNFproteins.
      SIZE:1026aminoacids;112480Da
      PTM:ThereareanumberofrepeatsofthetripeptideK-S-P,NFHisphosphorylatedonanumberoftheserinesinthismotif.ItisthoughtthatphosphorylationofNFHresultsintheformationofinterfilamentcrossbridgesthatareimportantinthemaintenanceofaxonalcaliber.&Phosphorylationseemstoplayamajorroleinthefunctioningofthelargerneurofilamentpolypeptides(NF-MandNF-H),thelevelsofphosphorylationbeingaltereddevelopmentallyandcoincidentwithachangeintheneurofilamentfunction.
      DISEASE:SwissProt:P12036#DefectsinNEFHareacauseofsusceptibilitytoamyotrophiclateralsclerosis(ALS)[MIM:105400].ALSisaneurodegenerativedisorderaffectingupperandlowermotorneurons,andresultinginfatalparalysis.Sensoryabnormalitiesareabsent.Deathusuallyoccurswithin2to5years.Theetiologyislikelytobemultifactorial,involvingbothgeneticandenvironmentalfactors.
      SIMILARITY:SwissProt:P12036##Belongstotheintermediatefilamentfamily.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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