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Millipore/MAB4703 | Anti-Caspase 3 Antibody, large subunit & proform, clone 4-1-18/MAB4703/100 µg
  • Millipore/MAB4703 | Anti-Caspase 3 Antibody, large subunit & proform, clone 4-1-18/MAB4703/100 µg

Millipore/MAB4703 | Anti-Caspase 3 Antibody, large subunit & proform, clone 4-1-18/MAB4703/100 µg

價(jià)格: ¥3816.00 市場價(jià): 6360.00

貨號: MAB4703
品牌: Millipore
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    • Description
      CatalogueNumberMAB4703
      Replaces04-439
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-Caspase3Antibody,largesubunit&proform,clone4-1-18
      AlternateNames
      • CPP32
      ProductInformation
      FormatPurified
      Control
      • POSITIVECONTROL:293(humanembryonickidney)celllysates
      PresentationPurifiedimmunoglobulinbyproteinA.LiquidinPBS,pH7.6,with0.1%sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainrefrigeratedat2-8°Cinundilutedaliquotsforupto12months.
      Applications
      ApplicationAnti-Caspase3Antibody,largesubunit&proform,clone4-1-18detectslevelofCaspase3&hasbeenpublished&validatedforuseinWB,IH(P).
      KeyApplications
      • WesternBlotting
      • Immunohistochemistry(Paraffin)
      ApplicationNotesWesternblot:1:1000.Theantibodywilldetectabandat32kDacorrespondingtotheinactiveunprocessedformofcaspase3aswellasa17-22kDabandcorrespondingtothelargesubunitofactivecaspase3.

      Immunohistochemistry(frozenandparaffin):1:500.



      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenRecombinantfull-lengthhumancaspase-3protein
      Epitopelargesubunit&proform
      Clone4-1-18
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityRecognizesthefulllength32kDacaspase-3protein.Highlyspecifictocaspase-3andshowsnocross-reactionwithothercaspasefamilymembers.Caspase-3isubiquitouslyexpressedandlikeothercaspasesissynthesizedasaninactiveproenzyme.Uponactivation,caspase-3iscleavedgeneratingtwosmallersubunitsof17kDaand12kDa.Caspase-3isoneofthemostextensivelystudiedenzymeinapoptosis.Activationofcaspase-3occursinresponsetoavarietyofapoptoticinducers.
      IsotypeIgG2aκ
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Thisproteincleavesandactivatescaspases6,7and9,andtheproteinitselfisprocessedbycaspases8,9and10.Itisthepredominantcaspaseinvolvedinthecleavageofamyloid-beta4Aprecursorprotein,whichisassociatedwithneuronaldeathinAlzheimer"sdisease.Alternativesplicingofthisgeneresultsintwotranscriptvariantsthatencodethesameprotein.
      GeneSymbol
      • CASP3
      • apopain
      • CASP-3
      • procaspase3
      • CPP32B
      • SCA-1
      • CPP-32
      • Apopain
      • CPP32
      • Yama
      • EC3.4.22.56[Contains:Caspase-3subunitp17
      • Caspase-3subunitp12].
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P42574#InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Attheonsetofapoptosisitproteolyticallycleavespoly(ADP-ribose)polymerase(PARP)ata"216-Asp--Gly-217"bond.Cleavesandactivatessterolregulatoryelementbindingproteins(SREBPs)betweenthebasichelix-loop-helixleucinezipperdomainandthemembraneattachmentdomain.Cleavesandactivatescaspase-6,-7and-9.Involvedinthecleavageofhuntingtin.
      SIZE:277aminoacids;31608Da
      SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya17kDa(p17)anda12kDa(p12)subunit.
      SUBCELLULARLOCATION:Cytoplasm.
      TISSUESPECIFICITY:Highlyexpressedinlung,spleen,heart,liverandkidney.Moderatelevelsinbrainandskeletalmuscle,andlowintestis.Alsofoundinmanycelllines,highestexpressionincellsoftheimmunesystem.
      PTM:CleavagebygranzymeB,caspase-6,caspase-8andcaspase-10generatesthetwoactivesubunits.Additionalprocessingofthepropeptidesislikelyduetotheautocatalyticactivityoftheactivatedprotease.Activeheterodimersbetweenthesmallsubunitofcaspase-7proteaseandthelargesubunitofcaspase-3alsooccurandviceversa.
      SIMILARITY:SwissProt:P42574##BelongstothepeptidaseC14family.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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