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Millipore/MAB4211 | Anti-CD34 Class I Antibody, clone B1-3C5/MAB4211/100 µg
  • Millipore/MAB4211 | Anti-CD34 Class I Antibody, clone B1-3C5/MAB4211/100 µg

Millipore/MAB4211 | Anti-CD34 Class I Antibody, clone B1-3C5/MAB4211/100 µg

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貨號(hào): MAB4211
品牌: Millipore
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    • Description
      CatalogueNumberMAB4211
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-CD34ClassIAntibody,cloneB1-3C5
      ProductInformation
      FormatPurified
      PresentationTheantibodyissuppliedinphosphatebufferedsaline,pH7.4,containing0.2%bovineserumalbuminand0.1%sodiumazide.Thecharacteristicsofeachlotaretestedbyelectrophoresisandflowcytometry.
      StorageandShippingInformation
      StorageConditionsForprolongedperiods,storebelow-20°Cinundilutedaliquots.AVOIDREPEATEDFREEZE/THAWCYCLES.Productstablefor6monthswhenstoredasdirected.

      WARNING:Themonoclonalreagentsolutioncontains0.1%sodiumazideasapreservative.Duetopotentialhazardsarisingfromthebuildupofthismaterialinpipes,spentreagentshouldbedisposedofwithliberalvolumesofwater.
      Applications
      ApplicationDetectCD34ClassIusingthisAnti-CD34Antibody(Class1),cloneB1-3C5validatedforuseinFC&IF.
      KeyApplications
      • FlowCytometry
      • Immunofluorescence
      ApplicationNotesIdentificationofCD34positivecellsisimportantforthediagnosisandclassificationofacutemyeloidleukemia(AML).Itisespeciallyusefulinpatientswithpoorlydifferentiatedmyeloblasts,aswellasinmyeloblasticcrisesofchronicgranulocyticleukemia.Ithasbeenrecommendedthatthisantibodybeusedaspartofapanelforscreeningofleukemia(Batinicetal.,1989).

      SUGGESTEDUSAGEDILUTION

      1.Flowcytometryandindirectimmunofluorescence1:25

      Dilutewithisotonicbuffer.Use50μlofdilutedantibodyper1x10E6peripheralbloodmononuclearcells(PBMC)orbonemarrowcellsin100μlofbuffer.

      2.Indirectimmunoperoxidasestaining-thefinaldilutionwilldependontheassayconditionsanddetectionsystememployed.However,adilutionofatleast1:25willbeapplicabletomostcommonlyusedsystems.
      BIOLOGicalInformation
      ImmunogenKG1humanacutemyelogenouscellline.
      CloneB1-3C5
      HostMouse
      SpecificityThisantibodybelongstoCD34(assignedbytheThirdInternationalWorkshoponLeukocyteDifferentiationAntigens,Oxford,1987;Hogg&Horton,1987).Itreactswitha120kDglycoproteinfoundonProgenitorcellpopulationsinhumanbonemarrowandfoetalliver(Katzetal.,1985).Thisantigenisnormallyrestrictedtoimmaturehumanhaemopoieticprecursorcells(Peschel&Koller,1989).

      Cellreactivity(Tindleetal.,1985):

      Normal:Noreactionwithnormalperipherallymphocytes,granulocytes,monocytes,erythrocytesandplatelets.Reactswith<4% of="" cells="" in="" normal="" bone="" marrow.="">

      Clinical:Reaction

      Acutemyeloidleukemia(AML,M1andM2)positive

      Nulllymphoblasticleukemia(NullALL)positive

      Commonacutelymphoblasticleukemia(CALL)positive

      Myeloblasticandlymphoblasticcrisesofchronic

      granulocyticleukemia(CGL)positive

      Chronicmyeloidleukemia(CML)negative

      Tcellacutelymphoblasticleukemia(TALL)negative

      Bcellacutelymphoblasticleukemia(BALL)negative

      Chroniclymphocyticleukemia(CLL)negative

      Celllines:

      ReactswithearlymyeloblastlineKG1.Noreactionwithnull,BorTlymphoid,erythroidorlatermyeloidcelllines.
      IsotypeIgG1
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryCD34isamonomericcellsurfaceantigenwithamolecularmassofapproximately110kDthatisselectivelyexpressedonhumanhematopoieticprogenitorcells.[suppliedbyOMIM]
      GeneSymbol
      • CD34
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P28906#PossIBLeadhesionmoleculewitharoleinearlyhematopoiesisbymediatingtheattachmentofstemcellstothebonemarrowextracellularmatrixordirectlytostromalcells.Couldactasascaffoldfortheattachmentoflineagespecificglycans,allowingstemcellstobindtolectinsexpressedbystromalcellsorothermarrowcomponents.Presentscarbohydrateligandstoselectins.
      SIZE:385aminoacids;40716Da
      SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
      TISSUESPECIFICITY:Selectivelyexpressedonhematopoieticprogenitorcellsandthesmallvesselendotheliumofavarietyoftissues.
      DEVELOPMENTALSTAGE:Onearlyhematopoieticprogenitorcells.
      PTM:Highlyglycosylated.&PhosphorylatedonserineresiduesbyPKC.
      DISEASE:SwissProt:P28906#AbnormalCD34expressioninleukemogenesis.
      SIMILARITY:SwissProt:P28906##BelongstotheCD34family.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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