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Millipore/MAB360 | Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5/MAB360/100 µL
  • Millipore/MAB360 | Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5/MAB360/100 µL

Millipore/MAB360 | Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5/MAB360/100 µL

價(jià)格: ￥3072.00 市場價(jià): 5120.00
貨號: MAB360
品牌: Millipore
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    • Description
      CatalogueNumberMAB360
      Replaces04-1031;04-1062
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-GlialFibrillaryAcidicProteinAntibody,cloneGA5
      AlternateNames
      • GFAP
      BackgroundInformationGlialfibrillaryacidicproteinisaclass-IIIintermediatefilament.GFAPisthemainconstituentofintermediatefilamentsinastrocytesandservesasacellspecificMarkerthatdistinguishesdifferentiatedastrocytesfromotherglialcellsduringthedevelopmentofthecentralnervoussystem.
      ProductInformation
      FormatAscites
      Control
      • PositiveControl:Culturedastrocytes,braintissue(whitematter),spinalchord,retina
      PresentationUnpurifiedmousemonoclonalIgG1liquidinbuffercontaining15mMsodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20oCfromdateofreceipt.
      HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationAnti-GlialFibrillaryAcidicProteinAntibody,cloneGA5detectslevelofGlialFibrillaryAcidicProtein&hasbeenpublished&validatedforuseinIC,IH,IH(P)&WBwithmorethan65productcitations.
      KeyApplications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry(Paraffin)
      • WesternBlotting
      ApplicationNotesImmunocytochemistry:
      Apreviouslotwasusedonculturedmammaliancells.

      Immunohistochemistry(paraffin):
      1:400-1:800dilutionfromapreviouslotwasusedonalcohol-fixedparaffinembeddedsectionsofratbrain(cerebrumorcerebellum)andhumanbrain.

      Immunoblotting:A1:1000dilutionofapreviouslotwasused.

      Optimalworkingdilutionsmustbedeterminedbytheenduser
      BIOLOGicalInformation
      ImmunogenPurifiedGFAPfromporcinespinalcord.
      CloneGA5
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityGlialfibrillaracidicprotein.Onwesternblotsofextractsfromahumangliomacellline(U33CG/343MG),MAB360recognizesabandatapproximately51kDacorrespondingtoGFAP(Debus,1983).ByimmunohistochemistryitrecognizesastrocytesandBergmanngliacells,gliomaandglialcellderivedtumors.Showsnocross-reactivitywithvimentin.
      IsotypeIgG1
      SpeciesReactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Pig
      • Rat
      • Rabbit
      SpeciesReactivityNoteReactswithBovine,Chicken,Human,Mouse,Porcine(Pig),RabbitandRat.Reactivitywithotherspecieshasnotbeendetermined.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesoneofthemajorintermediatefilamentproteinsofmatureastrocytes.Itisusedasamarkertodistinguishastrocytesfromotherglialcellsduringdevelopment.MutationsinthisgenecauseAlexanderdisease,araredisorderofastrocytesinthecentralnervoussystem.Anadditionaltranscriptvarianthasbeendescribed,butitsfulllengthsequencehasnotbeendetermined.
      GeneSymbol
      • GFAP
      • FLJ45472
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P14136#GFAP,aclass-IIIintermediatefilament,isacell-specificmarkerthat,duringthedevelopmentofthecentralnervoussystem,distinguishesastrocytesfromotherglialcells.
      SIZE:432aminoacids;49880Da
      SUBUNIT:Isoform3interactswithN-terminusofPSEN1.
      SUBCELLULARLOCATION:Cytoplasm.Note=Associatedwithintermediatefilaments.
      DISEASE:SwissProt:P14136#DefectsinGFAPareacauseofAlexanderdisease[MIM:203450].Alexanderdiseaseisararedisorderofthecentralnervoussystem.ItisaprogressiveleukoencephalopathywhosehallmarkisthewidespreadaccumulationofRosenthalfiberswhicharecytoplasmicinclusionsinastrocytes.Themostcommonformaffectsinfantsandyoungchildren,andischaracterizedbyprogressivefailureofcentralmyelination,usuallyleADIngtodeathusuallywithinthefirstdecade.InfantswithAlexanderdiseasedevelopaleukoencephalopathywithmacrocephaly,seizures,andpsychomotorretardation.Patientswithjuvenileoradultformstypicallyexperienceataxia,bulbarsignsandspasticity,andamoreslowlyprogressivecourse.
      SIMILARITY:SwissProt:P14136##Belongstotheintermediatefilamentfamily.
      MolecularWeight~51kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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