Application | UsemousemonoclonalAnti-BrdUAntibody,cloneBU-1,validatedinFlowCytometry,Immunocytochemistry,andImmunohistochemistryforthedetectionofbromodeoxyuridine(BrdU). |
KeyApplications | - FlowCytometry
- Immunocytochemistry
- Immunohistochemistry
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ApplicationNotes | AlthoughoriginallyreportedtostainnuclearDNABrdUincorporationinethanol-fixedcellswithoutacidorbasedenaturation,thispropertywaslaterattributedtotheDNaseactivitypresentinamycoplasma-contaminatedBU-1hybridomaculturesupernatantused(Gonchoroff,N.J.,etal.(1986).J.Immunol.Methods.93(1):97-101;Greipp,P.R.,etal.(1985).Am.J.Hematol.20(3):289-292).PleaseincludeanappropriateDNAdenaturationstepduringsamplepreparationpriortoimmunostainingusingthisproduct. |