當(dāng)前位置 ： Millipore >>> Millipore/MAB305 | Anti-Choline Acetyltransferase Antibody, clone 1E6/MAB305/100 µL
Millipore/MAB305 | Anti-Choline Acetyltransferase Antibody, clone 1E6/MAB305/100 µL
  • Millipore/MAB305 | Anti-Choline Acetyltransferase Antibody, clone 1E6/MAB305/100 µL

Millipore/MAB305 | Anti-Choline Acetyltransferase Antibody, clone 1E6/MAB305/100 µL

價(jià)格: ￥3072.00 市場(chǎng)價(jià): 5120.00
貨號(hào): MAB305
品牌: Millipore
規(guī)格
數(shù)量
庫(kù)存(0)
特別 提示
代購(gòu)產(chǎn)品：無(wú)質(zhì)量問(wèn)題不接受退換貨，下單前請(qǐng)仔細(xì)核對(duì)信息。
下單后請(qǐng)及時(shí)聯(lián)系客服核對(duì)商品價(jià)格，訂單生效后再付款。
資深產(chǎn)品顧問(wèn)
咨詢(xún)顧問(wèn)

全國(guó)免費(fèi)服務(wù)熱線(xiàn)

4000-520-616

  • 自營(yíng)商城 一站式服務(wù)
  • 廠(chǎng)家直采 剔除溢價(jià)
  • 品質(zhì)甄選 正品保證
  • 嚴(yán)控流程 只做188精品
  • 極速物流 如約送貨
  • 詳情
  • 使用說(shuō)明
  • 常見(jiàn)問(wèn)題
    • Description
      CatalogueNumberMAB305
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-CholineAcetyltransferaseAntibody,clone1E6
      AlternateNames
      • ChAT
      • CholineAcetylase
      • CHOACTase
      ProductInformation
      FormatAscites
      Control
      • Braintissue
      PresentationAscitesfluidcontainingnopreservatives.
      StorageandShippingInformation
      StorageConditionsMaintainfor1yearat-20°Cfromdateofshipment.Aliquottoavoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
      Applications
      ApplicationDetectCholineAcetyltransferaseusingthisAnti-CholineAcetyltransferaseAntibody,clone1E6validatedforuseinIH.
      KeyApplications
      • Immunohistochemistry
      ApplicationNotesImmunohistochemistry:1:100-1:250.Seeimmunohistochmistryprocedurebelow.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.

      IMMUNOHISTOCHEMISTRYPROCEDURE(PAPTECHNIQUE)FORMAB305,MONOCLONALANTIBODYTOCHOLINEACETYLTRANSFERASE

      I)Perfusion&SectioningProcedure

      1.Perfusethroughtheheartwithafixativesolutioncontaining4%paraformaldehydein0.12Mphosphatebuffer(pH7.3)forlightmicroscopy(LM),andadditionally,0.1%gluteraldehydeand.002%CaCl2forelectronmicroscopy(EM).

      2.Removebrainandpostfix2-18hoursat4°Cin4%paraformaldehydein0.12Mphosphatebuffer.

      3.Afterbrainisblockedforsectioning,washinseveralchangesofbufferfor2-3hours.

      4.SpecimensforEMaresectionedonaVibratome(50μm)andrinsedinbuffer,thoseforLMshouldbecryoprotectedin30%sucroseinbuffer.

      5.Afterfreezingwithdryice,30-40μmthicksectionsofLMspecimensarecutonacryostat.

      6.Sectionsarerinsed,andthenstoredinphosphatebuffercontaining0.1%sodiumazide.

      II)StainingProcedure

      Tissueisprocessedasfreely-floatingsectionsincontinuouslyagitatedsolutions.Allincubationsareperformedatroomtemperatureunlessotherwisestated.

      1.a.ForlocalizingChAT-positivesomataanddendrites:

      Sectionsarewashedin0.1MTris-bufferedsaline(TBS;containing1.4%NaCl,pH7.3)only.Nodetergentorenzymepretreatmentisused.

      b.ForlocalizingChAT-positiveterminal-likestructures:

      IncubatesectionsinTBS(pH8.1)for5minutesat37°C.TransfersectionstoTBS(pH8.1)containingpronase(1.2μg/mL)for11/2-2minutesat37°C,followedbyseveralicecoldbufferwashesforatotalof5minutes.Theconcentrationofpronaseandincubationtimeofthedigestionshouldbeevaluatedforeachregionexamined.

      c.ForlocalizingChATimmunoreactivityandsubsequentlycounterstainingthesections:

      IncubationinTBScontaining0.1%-0.8%TritonX-100for15minutesmayincreasethetissuepenetrationoftheImmunoReagents,butitalsoraisesthebackgroundstaining.

      2.Incubatesectionsinnormalgoatserum(3-5%)foronehour.Theworkingsolutionsofallantiserashouldalsocontainsimilarlydilutednormalgoatserum.

      3.Incubateinanti-ChATmonoclonalantibodysolution(Suggestedworkingdilution1:250,finalworkingdilutionmustbedeterminedbyenduser)for2hoursatroomtemperatureandthenforanadditional6-18hoursat4°C.

      4.Incubatewithsecondantibody(i.e.Goatanti-MouseIgG,Cat.No.:AP124,dilution1:50-100)for1-2hours.

      5.IncubatewithdilutedPAPcomplex(i.e.MousePAP,CatNo.:PAP14,conc.25-50μg/mL)foronehour.

      6.Afterrinsinginbuffer,thesecondantibodyandPAPstepsarerepeatedfor40minutesto1houreachinordertoamplifystainingintensity,particularlyofsmallChAT-containingstructures.

      7.Reactfor15minuteswith0.06%3,3"-diaminobenzidine×4HCl(DAB;dilutedinphosphatebufferedsaline,pH7.3)and0.006%H2O2.

      8.SpecimensforroutineLMarepostfixedfor1minutesin0.005%OsO4(osmiumtetraoxide),andthenmounted,dehydratedandcoverslipped.SelectedregionsblockedforEMarepostfixedin2%OsO4for1hour,enblocstainedwithuranylacetate,andflat-embeddedinEpon-Aralditeresin.
      BIOLOGicalInformation
      ImmunogenCholineacetyltransferasepurifiedfromratbrain.
      Clone1E6
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityRecognizescholinergicneuronsinthebrainandspinalcord(CNS).
      IsotypeIgG1
      SpeciesReactivity
      • Human
      • Monkey
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryCholinergicsystemsareimplicatedinnumerousneurologicfunctions.AlterationinsomecholinergicneuronsmayaccountforthedisturbancesofAlzheimerdisease.Theproteinencodedbythisgenesynthesizestheneurotransmitteracetylcholine.Alternativesplicevariantshavebeenfoundthatcontainalternative5"untranslatedexons.Threeofthefourdescribedsplicevariantsencodeidentical69kDaproteinswhileonevariantencodesboththe69kDaandalarger82kDaprotein.
      GeneSymbol
      • CHAT
      • ChAT
      • CMS1A2
      • CHOACTase
      • CMS1A
      • EC2.3.1.6
      PurificationMethodUnpurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P28329#CatalyzesthereversIBLesynthesisofacetylcholine(ACh)fromacetylCoAandcholineatcholinergicsynapses.
      SIZE:748aminoacids;82568Da
      DISEASE:SwissProt:P28329#DefectsinCHATarethecauseoffamilialinfantilemyastheniagravis2(FIMG2)[MIM:254210,254200];alsoknownasCMS-EA.FIMG2patientshavemyasthenicsymptomssincebirthorearlyinfancy,negativetestsforanti-AChRantibodies,andabruptepisodiccriseswithincreasedweakness,bulbarparalysis,andapneaprecipitatedbyundueexertion,fever,orexcitement.Inheritanceisautosomalrecessive.
      SIMILARITY:SwissProt:P28329##Belongstothecarnitine/cholineacetyltransferasefamily.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    螞蟻淘生物188，秉承螞蟻淘一貫的嚴(yán)謹(jǐn)態(tài)度，由螞蟻淘公司專(zhuān)業(yè)人員負(fù)責(zé)品控、采購(gòu)、物流、銷(xiāo)售、售后，保障正品優(yōu)質(zhì)。以“快速好省，為科研提供好產(chǎn)品、好價(jià)格”為理念，直接鏈接原廠(chǎng)家，從全國(guó)各地原制造商嚴(yán)格挑選188款科研精品，剔除品牌溢價(jià)，188生物新電商，把好的產(chǎn)品帶給科研！? 力求給你最優(yōu)質(zhì)的商品。
  • Q：生物188產(chǎn)品正品保障嗎？
    A：生物188質(zhì)量把控人員具有十年的從業(yè)經(jīng)驗(yàn)，在業(yè)界享有良好的口碑；自營(yíng)商城，直接從廠(chǎng)家采購(gòu)， 自己的團(tuán)隊(duì)負(fù)責(zé)國(guó)際物流和清關(guān)，中間沒(méi)有第三方，所有流程嚴(yán)格把控，100%保證正品，假一罰十。

    Q：下單后可以修改訂單嗎？
    A：下單后的商品付款之前可以修改；訂單付款成功，需要聯(lián)系我們客服進(jìn)行修改；客服電話(huà)：4000-520-616

    Q：可以開(kāi)發(fā)票嗎？
    A：本網(wǎng)站所售商品都是正規(guī)清關(guān)，均開(kāi)具16%正規(guī)發(fā)票，發(fā)票金額含配送費(fèi)金額，另有說(shuō)明的除外。

    Q：商品幾天可以發(fā)貨？
    A：生物188商品，全部現(xiàn)貨銷(xiāo)售，付款后即可發(fā)貨,一般一周內(nèi)送達(dá)！

    Q：如何聯(lián)系商家？
    A：有任何問(wèn)題夠可以電話(huà)咨詢(xún)我們，全國(guó)24小時(shí)免費(fèi)服務(wù)熱線(xiàn)：4000-520-616 或聯(lián)系我們的在線(xiàn)客服QQ：1570468124

    Q：收到的商品少了/發(fā)錯(cuò)了怎么辦？
    A：同個(gè)訂單購(gòu)買(mǎi)多個(gè)商品可能會(huì)分為一個(gè)以上包裹發(fā)出，可能不會(huì)同時(shí)送達(dá)，建議查看訂單詳情是否是 部分發(fā)貨狀態(tài)；如未收到，可聯(lián)系在線(xiàn)客服或者致電4000-520-616。

    Q：退換貨/維修需要多長(zhǎng)時(shí)間？
    A：一般情況下，退貨處理周期為客戶(hù)收到產(chǎn)品一個(gè)月內(nèi)（以快遞公司顯示簽收時(shí)間為準(zhǔn)），包裝規(guī)格、 數(shù)量、品種不符，外觀(guān)毀損、短缺或缺陷，請(qǐng)?jiān)谑盏截?4小時(shí)內(nèi)申請(qǐng)退換貨；特殊商品以合同條款為準(zhǔn)。

何為188

極簡(jiǎn)而嚴(yán)謹(jǐn)，我們僅銷(xiāo)售188款生物醫(yī)學(xué)科研用品，款款都是爆款；因?yàn)樯偎跃劢?，聚焦甄選每一款產(chǎn)品，聚焦服務(wù)每一位客戶(hù)!

關(guān)注我們 :

點(diǎn)擊QQ聯(lián)系我們
生物188微信

關(guān)注188微信公眾號(hào)

獲取最新優(yōu)惠活動(dòng)通知