Mouse monoclonal antibody raised against partial recombinant NHE-3.
Immunogen:
Recombinant fusion protein corresponding to C-terminus of Rabbit NHE-3.
Host:
Mouse
Reactivity:
Rabbit, Rat
Specificity:
This antibody does not show any cross-reactivity with any other NHE isoforms.
Form:
Liquid
Isotype:
IgG1
Quality Control Testing:
Antibody Reactive Against Recombinant Protein.
Recommend Usage:
Western Blot (0.5 ug/mL)The optimal working dilution should be determined by the end user.
Storage Buffer:
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction:
Store at 4°C for short term. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
Download
Publication Reference
1.
Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites.Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS.Am J Physiol Renal Physiol. 2005 Aug;289(2):F249-58. Epub 2005 Feb 1.
2.
Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney.Biemesderfer D, Rutherford PA, Nagy T, Pizzonia JH, Abu-Alfa AK, Aronson PS.Am J Physiol. 1997 Aug;273(2 Pt 2):F289-99.
Applications
Western Blot (Tissue lysate)
Detection of NHE-3 in rat kidney lysate using NHE-3 monoclonal antibody, clone 19F5 (Cat # MAB2324).