ApplicationNotes | Inhibitionofcellattachment:inhibitsSCC9celladhesiontohumanlamininandlamininGD-2peptide,butnottoEHSlaminin,typeIVcollagenorfibronectin.DoesnotinhibitSKOV-3celladhesiontolaminin,fibronectinortypeIVcollagen.Immunohistochemistry:onacetone-fixedfrozenhumanepithelialtissuesincludingtongue,foreskinandovary.Notforuseonformalin-fixedtissue.Flowcytometry:stainshumansquamouscellcarcinoma(SCC9),ovariancarcinoma(SKOV-3)andumbilicalveinendothelium(HUVEC).StainsRKOorK562cellstransfectedforCD104expression.Doesnotstainmonocytes,neutrophils,lymphocytes,Tcells,Bcellsorplatelets.Immunoprecipitation:precipitatesPAGEbandsof66,76,120,135and200kDa(reduced)or66,110,125,130and190kDa(non-reduced)fromsurface-biotinylatedSCC9cells.Workingdilutionsmustbedeterminedbyenduser. |