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Abnova/CD45RA monoclonal antibody, clone SPM504/1kit/MAB13150
  • Abnova/CD45RA monoclonal antibody, clone SPM504/1kit/MAB13150

Abnova/CD45RA monoclonal antibody, clone SPM504/1kit/MAB13150

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貨號(hào): MAB13150
品牌: Abnova
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  • 詳情
  • 使用說(shuō)明
  • 常見(jiàn)問(wèn)題
      • Specification
      • Gene Info
      • Pathway
      • Disease
      • Related Products
      • List All
      • Specification
      • Product Description:
      • Mouse monoclonal antibody raised against native human CD45RA.
      • Immunogen:
      • Stimulated human leukocytes.
      • Host:
      • Mouse
      • Theoretical MW (kDa):
      • 205-220
      • Reactivity:
      • Human
      • Specificity:
      • Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA (Workshop III). CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T cells, 50% of peripheral CD8+ T cells, B cells, and leukemic B cell lines. T cells expressing CD45RA are naive or virgin T cells. T cells expressing CD45RO are memory T cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T cells. This monoclonal antibody is useful in study on the subpopulation of CD4+ or CD8+ T cells. It can especially be used to differentiate T cell lymphomas (CD45RO+ve) from B cell lymphomas (CD45RA+ve).
      • Form:
      • Liquid
      • Purification:
      • Protein A/G purification
      • Isotype:
      • IgG1, kappa
      • Recommend Usage:
      • Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)Immunofluorescence (1-2 ug/mL)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)The optimal working dilution should be determined by the end user.
      • Storage Buffer:
      • In 10 mM PBS.
      • Storage Instruction:
      • Store at -20 to -80°C.Aliquot to avoid repeated freezing and thawing.
      • Datasheet:
      • PDF DownloadDownload
      • Applications
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with CD45RA monoclonal antibody, clone SPM504 (Cat # MAB13150).
      • Immunofluorescence
      • Flow Cytometry
      • Application Image
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      • enlarge
      • Immunofluorescence
      • Flow Cytometry
      • Gene Information
      • Entrez GeneID:
      • 5788
      • Gene Name:
      • PTPRC
      • Gene Alias:
      • B220,CD45,CD45R,GP180,LCA,LY5,T200
      • Gene Description:
      • protein tyrosine phosphatase, receptor type, C
      • Omim ID:
      • 126200, 151460, 609532
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq
      • Other Designations:
      • CD45 antigen,T200 glycoprotein,T200 leukocyte common antigen,glycoprotein,leukocyte-common antigen,protein tyrosine phosphatase, receptor type, c polypeptide
      • Gene Pathway
      • Cell adhesion molecules (CAMs)
      • Fc gamma R-mediated phagocytosis
      • Primary immunodeficiency
      • T cell receptor signaling pathway
      • Related Disease
      • Arthritis, Juvenile Rheumatoid
      • Arthritis, Rheumatoid
      • Ascariasis
      • Autoimmune Diseases
      • Cardiomyopathy, Dilated
      • Diabetes Mellitus, Type 1
      • Genetic Predisposition to Disease
      • Graves Disease
      • Hashimoto Disease
      • Hepatitis B
      • Hepatitis C
      • Hepatitis, Autoimmune
      • HIV Infections
      • Inflammatory Bowel Diseases
      • Lupus Erythematosus, Systemic
      • Lymphopenia
      • Multiple Sclerosis
      • Paraparesis, Tropical Spastic
      • Scleroderma, Systemic
          • ... see more
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