9° N™ DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands that are hybridized and accurately paired, with no gap, to a complementary DNA strand. 9° N DNA Ligase uses ATP as a cofactor and it is active at elevated temperatures (45° C – 70° C).
Product Source
Purified from anE. colistrain containing the cloned ligase gene from the extremely thermophilic marine archaeaThermococcussp.(strain 9°N). The archaea was isolated from a submarine thermal vent, at a depth of 2,500 meters, 9° north of the equator at the East Pacific Rise (1).
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DNA Ligases Products
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