產(chǎn)品概述:產(chǎn)品內(nèi)容:
組分 | L6023S(30T) | L6023M(150T) | L6023L(300T) |
A: Calcein AM(4 mM in anhydrous DMSO) | 10 μL | 50 μL | 100 μL |
B: Ethidium homodimer-1(EthD-I)(2 mMin DMSO/H2O 1:4(v/v)) | 30 μL | 150 μL | 300 μL |
注:本試劑盒次數(shù)是按照流式細(xì)胞儀一個(gè)樣品使用0.5 mL 工作液規(guī)定的。光譜信息:Calcein AM:Ex/Em= 494/517 nm;EthD-I:Ex/Em = 528/617 nm(結(jié)合DNA)儲(chǔ)存條件-20?C避光保存。注意Calcein AM容易水解,需密封干燥保存,稀釋工作液需當(dāng)天配制。有效期見(jiàn)外包裝。產(chǎn)品介紹Live & DeadTM動(dòng)物細(xì)胞活力/毒性檢測(cè)試劑盒(Calcein AM, EthD-1)是一種為動(dòng)物細(xì)胞死活檢測(cè)提供雙熒光染色的試劑盒。試劑盒內(nèi)的兩種探針可分別測(cè)定細(xì)胞內(nèi)酯酶活性和質(zhì)膜完整性從而反映細(xì)胞活力。本試劑盒可用于熒光顯微鏡、流式細(xì)胞儀、酶標(biāo)儀以及其他熒光檢測(cè)系統(tǒng)。Live & DeadTM動(dòng)物細(xì)胞活力/毒性檢測(cè)試劑盒(Calcein AM, EthD-1)可以應(yīng)用于大多數(shù)的真核哺乳動(dòng)物細(xì)胞,包括貼壁細(xì)胞核的某些組織,但不適用于真菌和酵母。該試劑盒與相同用處的臺(tái)盼藍(lán)相比,更快捷安全且靈敏度更高。
說(shuō)明書(shū):UE-L6023S/L6023M/L6023L MSDS:MSDS L6023 Live & DeadTMViabilityCytotoxicity Assay Kit for Animal Cells (Calcein AM, EthD-1)
常見(jiàn)問(wèn)題解答:◆你們家有兩款哺乳動(dòng)物死活染色試劑盒,分別是CalceinAM,PI(貨號(hào)L6037)與CalceinAM,EthD-1(貨號(hào)L6023,這兩者的區(qū)別是什么?EthD-1相較于PI來(lái)說(shuō),對(duì)DNA的親和力更高,低濃度下即可滿(mǎn)足染色效果。◆我如何為L(zhǎng)IVE/DEAD細(xì)胞活力實(shí)驗(yàn)準(zhǔn)備死細(xì)胞對(duì)照?
有兩種簡(jiǎn)單的方法。一種是通過(guò)60°C放置20分鐘熱滅活細(xì)胞。第二種是將細(xì)胞放入70%乙醇。乙醇固定的細(xì)胞可以在冰箱中無(wú)限期儲(chǔ)存直到使用,可達(dá)好幾年。步驟:a.離心細(xì)胞、使細(xì)胞沉淀并去除上清b.固定細(xì)胞:在15mL含有細(xì)胞團(tuán)的試管中加入10 mL70%冰乙醇,先開(kāi)始逐滴加入后震蕩,混合均勻。c.在冰箱中儲(chǔ)存直到使用。d.快使用時(shí),水洗兩次且在選擇的緩沖液中重懸。
◆用該試劑盒染色后,可以進(jìn)行后續(xù)固定步驟嗎?染色后不可以進(jìn)行固定步驟。PI作為膜非透性染料,固定后將轉(zhuǎn)移到所有細(xì)胞,不再維持死細(xì)胞特異性染色。 使用本產(chǎn)品的文獻(xiàn):1.Regional and sustained dual-release of growth factors from biomimetictri-layered scaffolds for the repair of large-scale osteochondral defectsYunsheng Dong,Xun Sun,Zhiling Zhang,Yufei Liu,Lin Zhang,Xiangyun Zhang,Ying Huang,Yanhong Zhao,Chunxiao Qi,Adam C.Midgley,Shufang Wang,Qiang YangApplied materials today(Volume 19, June 2020, 100548)應(yīng)用方向:仿生三層支架上hUCMSCs細(xì)胞死活染色2.PEG Linker Improves Antitumor Efficacy and Safety of Affibody-Based Drug ConjugatesQiyu Li,Wenjing Li,Keyuan Xu,Yutong Xing,Haobo Shi,Zhe Jing,Shuang Li,Zhangyong HongInternational Journal of Molecular Sciences3.Multifunctional Injectable Hydrogel Loaded with Cerium-Containing Bioactive Glass Nanoparticles for Diabetic Wound HealingYue-Hua Chen,Zhou-Feng Rao,Yu-Jie Liu,Xiang-Sheng Liu,Yu-Fei Liu,Lan-Ju Xu,Ze-Qi Wang,Jing-Yue Guo,Lin Zhang,Yun-Sheng Dong,Chun-Xiao Qi,Chao Yang,Shu-Fang WangBiomolecules 2021, 11(5), 702參考文獻(xiàn):1.Visualization of keratocytes in the human cornea with fluorescence microscopy應(yīng)用方向:角膜組織切片染色2.Effect of matrix metalloproteinases activity on outflow in perfused human organ culture應(yīng)用方向:死活細(xì)胞染色:評(píng)估培養(yǎng)物生存力3.Metabolizing enzyme toxicology assay chip (MetaChip) for high-throughput microscale toxicity analyses應(yīng)用方向:死活細(xì)胞染色:高通量微量毒性分析4.Quantification of effector/target conjugation involving natural killer (NK) or lymphokine activated killer (LAK) cells by two-color flow cytometry應(yīng)用方向:流式細(xì)胞儀分析死活細(xì)胞能力