The antiserum was produced against synthesized peptide derived from human PARP.
Immunogen Range
196-245
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
PARP (Cleaved-Gly215) Antibody detects endogenous levels of fragment of activated PARP resulting from cleavage adjacent to Gly215.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PARP1
Database Links
Gene ID: 142 Swiss-Prot #: P09874
OMIM #: 173870 Unigene #: Hs.177766
Target Modification
Cleaved
Modification Sites
Application Images
Image 1
Western blot analysis of lysates from NIH/3T3 cells, treated with etoposide 25uM 1h, using PARP (Cleaved-Gly215) Antibody. The lane on the right is blocked with the synthesized peptide.