The antiserum was produced against synthesized peptide derived from human Caspase 3.
Immunogen Range
126-175
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:20000
Specificity
Caspase 3 (p17,Cleaved-Asp175) Antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CASP3
Database Links
Gene ID: 836 Swiss-Prot #: P42574
OMIM #: 600636 Unigene #: Hs.141125
Target Modification
Cleaved
Modification Sites
Asp175
Application Images
Image 1
Western blot analysis of lysates from COLO cells, using Caspase 3 (p17,Cleaved-Asp175) Antibody. The lane on the right is blocked with the synthesized peptide.