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Pierce/ERK1/ERK2 (Phospho) [pT185/pY187] Human ELISA Kit/96 tests/KHO0091
  • Pierce/ERK1/ERK2 (Phospho) [pT185/pY187] Human ELISA Kit/96 tests/KHO0091

Pierce/ERK1/ERK2 (Phospho) [pT185/pY187] Human ELISA Kit/96 tests/KHO0091

價格: ￥5988.00 市場價: 9980.00
貨號: KHO0091
品牌: Pierce
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    • Description

      TheERK1/2[pTpY185/187]ELISAresearchuseonlykitisdesignedtodetectandquantifythelevelofERK2proteinphosphorylatedatthreonineresidue185andtyrosine187andERK1atthreonine202andtyrosine204.ThisassayisintendedforthedetectionofERK1/2[pTpY185/187]fromhumancelllysatesusing96-wellplatesandamicroplatereader.

      •Easytorun
      •Moresensitivethanwesterns
      •Convenientprecoated,removable8-wellstrips

      Easy-to-RunSandwichELISA
      Thetotalassayincubationtimeisonly4hours.AmonoclonalcaptureantibodyspecificforERK1/2hasbeencoatedontothewellsofthe96-wellplateprovided.Duringthefirstincubation,standardsofknownERK1/2[pTpY185/187]contentandunknownsamplesarePipettedintothewellsandtheERK1/2antigenbindstotheimmobilized(capture)antibody.Afterwashing,arabbitantibodyspecificforERK1/2[pTpY185/187]isaddedtothewells.Duringthesecondincubation,thisantibodyservesasadetectionantibodybybindingtotheimmobilizedERK1/2proteincapturedduringthefirstincubation.Afterwashing,ahorserADIshperoxidase–labeledanti–rabbitIgGisadded.Thisbindstothedetectionantibodytocompletethefour–membersandwich.Afterathirdincubationandwashingtoremovealltheunboundenzyme,asubstratesolution(TMB)isadded,whichisacteduponbytheboundenzymetoproducecolor.TheintensityofthiscoloredproductisdirectlyproportionaltotheconcentrationofERK1/2[pTpY185/187]presentintheoriginalspecimenandtheopticaldensitycanbereadonastandardmicroplatereader.

      DetectLow-ExpressingProteinsandUseLessSample
      ELISAkitsdesignedtomeasureintracellularsignalingtargetsare2–10timesmoresensitivethanwesternblotting.Theimprovedsensitivityenablesyoutodetectlow-expressingproteinsthatotherwisemaynotbedistinguishablefrombackground.Inaddition,theamountofsampleneededtoruntheassayislessthanwhatisneededforwesternblots.

      Ready-to-UseERK1/2[pTpY185/187]ELISAKitContainsTheseReagents,Buffers,andSupplies:
      •ERK1/2AntibodyCoated96-WellPlate
      •ERK1/2[pTpY185/187]Standard
      •StandardDiluentBuffer
      •ERK1/2[pTpY185/187]DetectionAntibody
      •Anti-RabbitIgG-HRP(100X)
      •HRPDiluent
      •WashBufferConcentrate(25X)
      •StABIlizedChromogen,TMB
      •StopSolution
      •PlateCovers
      •Detailedprotocolwithvalidationtests

      ConvenientKitFormat
      Thekitcomeswithacaptureantibodyprecoatedtotheplate,eliminatinganovernightcoatingprocess.Theplatesaremachinecoatedtoprovidelowvariabilitywell-to-well.FurThermore,theplatecomesasremovable8-wellstripsallowingyoutorunasfewsamplesasyouwant.Inaddition,thestandardsprovidedinthekitcanbeusedtogetquantitativeresultsoffthestandardcurveand/orcanbeusedasapositivecontrol.

      RigorousValidation
      EachELISAkitisvalidatedforsensitivity,specificity,precision,andlot-to-lotconsistency.Seeprotocolinsertformoreinformationonvalidation.

      PartoftheELISAKitFamily
      WehaveawidemenuofELISAresearchuseonlykits.Withexcellentsensitivity,provenspecificity,andconsistentperformance,theseoptimizedELISAkitsofferyouachoicewhenitcomestogettingphysiologicallyrelevantandquantitativeresults.

      Forresearchuseonly.Notintendedforhumanoranimaltherapeuticordiagnosticuse.

      RelatedLinks:

      LearnmoreaboutELISAKits
      LearnmoreaboutotherImmunoassays
      ForResearchUseOnly.Notforuseindiagnosticprocedures.

      Figures

      Specifications

      ProteinFamily:Kinases&Inhibitors
      AssayIncubationTime:4hr
      AssayRange:1.6U/mL
      AssaySensitivity:<0.8Units/mL
      DetectionMethod:Colorimetric
      ForUseWith(Equipment):MicroplateReader
      FormerIdentifiers:Novex
      GeneAliases:ERK,ERK-2,ERK2,ERT1,MAPK2,P42MAPK,PRKM1,PRKM2,p38,p40,p41,p41mapk,p42-MAPK, ERK-1,ERK1,ERT2,HS44KDAP,HUMKER1A,P44ERK1,P44MAPK,PRKM3,p44-ERK1,p44-MAPK
      GeneID:5594, 5595
      GeneName:mitogen-activatedproteinkinase1, mitogen-activatedproteinkinase3
      GeneSymbol:MAPK1, MAPK3
      ImmunoassayKitFormat:CoatedELISAKit
      LabelorDye:HRP(HorseradishPeroxidase)
      ProductSize:96tests
      ProteinName:Mitogen-activatedproteinkinase1, Mitogen-activatedproteinkinase3
      ProteinSubtype:Serine⁄ThreonineKinases
      SampleType(Specific):CellExtracts
      SampleVolume:100µL
      ShippingCondition:WetIce
      Target(KitNamed):ERK1/ERK2
      TargetSite-Specificity:[pT185/pY187]
      TargetSpecies(Named):Human
      TargetSpecies(Validated):Human, Mouse, Rat
      TargetSub-Specificity:Phospho
      UniProtID:P27361, P28482

      Contents&storage

      Pre-coatedplate(s),standardorcalibrator,detectorantibody,HRPconjugate,diluents,washbuffer,substrate(s),andstopsolution.Storekitat2-8°C.Seeproductmanualfordetailedcontentsandstorageconditionsformaximumstability.
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