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Pierce/Amyloid beta 42 Human ELISA Kit, Ultrasensitive/96 tests/KHB3544
  • Pierce/Amyloid beta 42 Human ELISA Kit, Ultrasensitive/96 tests/KHB3544

Pierce/Amyloid beta 42 Human ELISA Kit, Ultrasensitive/96 tests/KHB3544

價(jià)格: ¥7800.00 市場價(jià): 13000.00

貨號: KHB3544
品牌: Pierce
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    • Description

      TheHumanBetaAmyloid[1-42]Ultrasensitive(Aβ42US)ELISAresearch-use-onlykitistobeusedforthequantitativedeterminationofHumanAβ42insamples(e.g.,cellculturesupernatants,tissuehomogenates,cerebrospinalfluid[CSF],etc.)using96-wellplatesandamicroplatereader.TheassayrecognizesbothnaturalandsyntheticformsofhumanAβ42.Theanti-humanAβ42antibodyusedinthiskitiscapableofselectivelydetectingAβ42andnotAβ40/Aβ43.*

      Performancecharacteristics
      •Sensitivity:<1>
      •Standardcurverange:1.56–100pg/mL
      •Sampletypes:cellculturesupernatants,tissuehomogenates,cerebrospinalfluid(CSF)
      •Speciescross-reactivity:human
      •Samplevolume:50μL(prediluted)
      •Totalassayincubationtime:4hrs

      Principleofthemethod
      TheHumanAβ42USkitisasolidphasesandwichenzyme-linkedimmunosorbentassay(ELISA).AmonoclonalantibodyspecificfortheNH2-terminusofhumanAβhasbeencoatedontothewellsofthemicrotiterstripsprovided.Samples,includingstandardsofknownhumanAβcontent,controlspecimensandunknownsarePipettedintothesewells,andco-incubatedwitharabbitmonoclonalantibody*specificfortheCOOH-terminusofthe1-42Aβsequence.ThisCOOH-terminalsequenceiscreateduponcleavageoftheanalyzedprecursor.

      BoundrabbitantibodyisdetectedbytheuseofahorserADIshperoxidase-labeledanti-rabbitantibody.Afterremovalofexcessanti-rabbitantibody,asubstratesolutionisadded,whichisacteduponbytheboundenzymetoproducecolor.TheintensityofthiscoloredproductisdirectlyproportionaltotheconcentrationofhumanAβ42presentintheoriginalspecimen.

      Backgroundinformation
      Alzheimer’sDisease(AD)ischaracterizedbythepresenceofextracellularplaquesandintracellularneurofibrillarytangles(NFTs)inthebrain.Themajorproteincomponentoftheseplaquesisβamyloidpeptide(Aβ),a40to43aminoacidpeptidecleavedfromamyloidprecursorproteinbyβ-secretase(i.e.,BACE)andaputativeγ(gamma)secretase.IncreasedreleaseofthelongerformsofAβpeptide,Aβ42orAβ43,whichhaveagreatertendencytoaggregatethanAβ40,occursinindividualsexpressingcertaingeneticmutations,expressingcertainApoEalleles,ormayinvolveother,stillundiscovered,factors.ManyresearcherstheorizethatitisthisincreasedreleaseofAβ42/Aβ43thatleadstotheabnormaldepositionofAβandtheassociatedneurotoxicityinthebrainsofaffectedindividuals.

      EachHumanAβ42USELISAkitisvalidatedforsensitivity,specificity,precision,andlot-to-lotconsistency.Seeproductinsertformoreinformationonvalidation.

      Relatedlinks
      LearnmoreaboutELISAkits
      Learnmoreaboutotherimmunoassays

      *PleaserefertotheELISAprotocolforspecificreferencecitations.
      ForResearchUseOnly.Notforuseindiagnosticprocedures.

      Figures

      Specifications

      AssayIncubationTime:4hr
      AssayRange:1.56-100pg/mL
      AssaySensitivity:<1pg/mL
      DetectionMethod:Colorimetric
      ForUseWith(Equipment):MicroplateReader
      FormerIdentifiers:Novex
      GeneAliases:AAA,ABETA,ABPP,AD1I,CTFgamma,CVAP,PN-II,PN2,APP
      GeneID:351
      GeneName:amyloidbetaprecursorprotein
      GeneSymbol:APP
      ImmunoassayKitFormat:CoatedELISAKit
      ImmunoassayKitGrade:Ultrasensitive
      LabelorDye:HRP(HorseradishPeroxidase)
      ProductSize:96tests
      ProteinName:AmyloidbetaA4protein
      SampleType(Specific):CerebrospinalFluid
      SampleVolume:50µL
      ShippingCondition:WetIce
      Target(KitNamed):Amyloidbeta42
      TargetSpecies(Named):Human
      TargetSpecies(Validated):Human
      UniProtID:P05067

      Contents&storage

      Pre-coatedplate(s),standardorcalibrator,detectorantibody,HRPconjugate,diluents,washbuffer,substrate(s),andstopsolution.Storekitat2-8°C.SeeproductmanualfordetailedcontentsandstorageconditionsformaximumstABIlity.
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