ProductBackground:ThePI(4)PMassassayisacompetitiveELISAinwhichthesignalisinverselyproportionaltotheamountofPI(4)Pmeasured.ThePI(4)PextractionsareaddedtothecoloredmixingplatewithPI(4)PGrip.ThismixtureisthentransferredtothePI(4)PDetectionPlateforcompetitivebinding.Aperoxidase-linkedsecondarydetectorandcolorimetricdetectionisusedtodetecttheamountofPI(4)PGripbindingtotheplate.Theassayissensitiveto0.7pmolPI(4)P. StorageThekitcomesintwopartswithdifferentstoragerequirements.UponreceiptstoreKitPart1at4°CandKitPart2at-20°C.Storepreparedreagentsasindicatedintheprotocol.FeaturedinReferences1)S.Choi,etal."AgoNIST-stimulatedphosphatidylinositol-3,4,5-trisphosphategenerationbyscaffoldedphosphoinositidekinases"NatureCellBiology(2016)18(12),1324-35,doi:10.1038/ncb3441RelatedProductsEchelon'sPIPMassAssays PIP3MassELISA PI(4,5)P2MassELISA PI(3,4)P2MassELISA PI(3)PMassELISA PIK-93(PI4KIIIbinhibitor) |