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Millipore/IHCR2025-6 | IHC Select? Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3/IHCR2
  • Millipore/IHCR2025-6 | IHC Select? Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3/IHCR2

Millipore/IHCR2025-6 | IHC Select? Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3/IHCR2

價格: ¥2580.00 市場價: 4300.00

貨號: IHCR2025-6
品牌: Millipore
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    • Description
      CatalogueNumberIHCR2025-6
      TradeName
      • IHCSelect
      • Chemicon
      DescriptionIHCSelect?Anti-CytokeratinAE1/AE3(Pancytokeratins)Antibody,prediluted,cloneAE1/AE3
      OverviewKeratinsareagroupofwater-insolubleproteinsthatformmonofilaments,aclassofintermediatefilament.Thesefilamentsformpartofthecytoskeletalcomplexinepidermisandinmostotherepithelialtissues.Nineteenhumanepithelialkeratinsareresolvedwithtwo-dimensionalgelselectrophoresis(Moll,R.,1982).Thesecanbedividedintoacid(pI<5.7) and="" basic="" (pi="">6.0)subfamilies.Theacidickeratinshavemolecularweightsof56.5,55,51,50,50",48,46,45,and40kD.Thebasickeratinshavemolecularweightsof65-67,64,59,58,56,and52kD.Membersoftheacidicandbasicsubfamiliesarefoundtogetherinpairs.Thecompositionofkeratinpairsvarieswiththeepithelialcelltype,stageofdifferentiation,cellulargrowthenvironment,anddiseasestate(Sun,T.T.,1984;Cooper,D.,1985;Sun,T.T.,1985).The56.5/65-67kDpairispresentinkeratinized(differentiated)epidermis.The55/64kDpairischaracteristicofnormal(corneal-type)epithelialdifferentiation(Moll,R.,1982;Sun,T.T,1984).The51/59kDpairischaracteristicofthestratifiedsquamousepithelialofinternalorganismsuchasesophagusandtongue(Moll,R.,1982;Cooper,D.,1985).The51/58kDpairisakeratinocyteMarker;thispairispresentinalmostallstratifiedepitheliairrespectiveofthestateofcellularstratification(Moll,R.,1982;Sun,T.T.,1984).The48/56kDpairischaracteristicofhyper-proliferative(de-differentiated)keratinocytes(Moll,R.,1982;Weiss,R.A.,1984).The45/52kDpairandtoalesserextent,the46/54kDpairarecharacteristicofsimpleepithelia(Moll,R.,1982).The40kDkeratinispresentinmostepitheliaexceptadultepidermis(Moll,R.,1982).
      ProductInformation
      FormatPurified
      PresentationPurifiedmonoclonalmouseantibodysuppliedinliquidformatdilutedinPBS,pH7.2withstABIlizers,0.2%Tween20,and0.1%ProClin300aspreservative.
      StorageandShippingInformation
      StorageConditionsMaintainat2-8°C.Refertovialforexpirationdating.
      Applications
      ApplicationIHCSelectAnti-CytokeratinAE1/AE3(Pancytokeratins)Antibody,prediluted,cloneAE1/AE3isanantibodyagainstCytokeratinAE1/AE3(Pancytokeratins)foruseinIH(P).
      KeyApplications
      • Immunohistochemistry(Paraffin)
      ApplicationNotesAntibodyispredilutedandreadytouseforImmunohistochemistryofformalin-fixed,paraffin-embeddedtissues.

      Pretreatment:HeatInducedEpitopeRetrieval(HIER).RecommendCitrateBuffer,pH6.0(Cat.No.21545).NosignalwasdetectedwithoutEpitoperetrieval.

      Incubation:10minuteswithIHCSelect?DetectionKits.

      CytokeratinAE1/AE3hasbeenpredilutedforuseastheprimaryantibodywithChemicon"sIHCSelect?DetectionKitsandProtocols(CatalogNos.DAB050,DET-HP1000,APR050,andDET-APR1000),butothersupplier"sIHCdetectionsystemsmaybeused.Foroptimizedprotocoldetails,visitwww.chemicon.comandselecttheprotocolslinkunderCat.No.IHCR2025-6.
      BIOLOGicalInformation
      ImmunogenHumanepidermalkeratin
      CloneAE1/AE3
      HostMouse
      SpecificityAnti-KeratinAE1recognizesthe56.5,50,50",48,and40kDkeratinsoftheacidicsubfamily.Anti-keratinAE3recognizesallmembersofthebasicsubfamily.AE1/AE3reactswithbothacidicandbasickeratins.Stainingislocalizedtothecytoplasm.AE1/AE3specificallybindstoantigenslocatedinthecytoplasmofnormalepithelia.Reactswithcellsofepithelialoriginincludingsimpleandstratifiedepitheliaandepidermis.
      IsotypeIgG1
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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