Properties:
Related Categories |
Analytical/Chromatography,
GE Healthcare Life Sciences Protein Sample Preparation Formats,
HiTrap & PD-10 Cartridges,
HiTrap System/syringe,
Molecular Biology,
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shelf life
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Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
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packaging
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pack of 5 × 1 mL
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mfr. no.
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GE Healthcare, 17-0402-01
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parameter
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flow rate
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<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
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42 psi
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bed size
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7 mm × 25 mm
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bed volume
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1 mL
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column I.D.
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7 mm
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matrix
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highly cross-linked 6% agarose
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avg. part. size
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34 μm
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cleaning
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2 - 10 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
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working range
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3 - 9
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storage temp.
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2-8°C
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Description:
Analysis Note
To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.
Features and Benefits
• High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
• 34 μm bead size for high-performance, high-resolution purifications.
• Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC™ System.
• Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
• Robust, easy-to-use method.
General description
HiTrap® Protein A HP is a prepacked Protein A Sepharose® High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.
HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.
Legal Information
FPLC is a trademark of GE Healthcare
HiTrap is a registered trademark of GE Healthcare
Sepharose is a registered trademark of GE Healthcare
Safety:
Symbol
GHS02
Precautionary statements
P210-P370 + P378
RIDADR
UN1170 - class 3 - PG 3 - Ethanol, solution