Properties:
RelatedCategories | AffinityChromatography,Analytical/Chromatography,GEDesaltingColumns,GelFiltrationChromatography,HPLC, |
shelflife | Pleasebeawarethisproductmaybeshipped90daysbeforetheexpirationdate.Formoreinformationonthebatchspecificexpirationdate,pleasecontacttechnicalservice. |
packaging | packof5×1 mL |
mfr.no. | GEHealthcare,11-0004-58 |
parameter | flowrate |
| <4mL/minflowrate(Thepressureoverthepackedbedvariesdependingonarangeofparameterssuchasthecharacteristicsofthechromatographymediumandthecolumntubingused.) |
| <4ml/minflowrate(1)Thepressureoverthepackedbedvariesdependingonarangeofparameterssuchasthecharacteristicsofthechromatographymediumandthecolumntubingused.) |
| 42psi(H2Oatroomtemperature.) |
bedsize | 7 mm×25 mm |
bedvolume | 1 mL |
columnI.D. | 7 mm |
matrix | 6%cross-linkedagarose |
particlesize | 45-165 μm |
averagediameter | 90 μm |
cleaning | 2-14(Ni2+-strippedmedium.) |
workingrange | 3-12(Ni2+-strippedmedium.) |
capacity | ~40 mgbindingcapacity(histidine-taggedprotein/mlmedium)(Proteinbindingcapacityisprotein-to-proteindependent.) |
| ~40 mgbindingcapacity(histidine-taggedprotein) |
suitABIlity | suitableforbioprocessmedium |
Description:
AnalysisNote
ToviewtheCertificateofAnalysisforthisproduct,pleasevisitwww.geLifesciences.com.
FeaturesandBenefits
• Optimizedforpurificationofhistidine-taggedproteinsdirectlyfromhomogenized,unclarifiedcelllysates.
• Highbindingcapacity,approx.40mg/mLmediumandnegligIBLeNi2+leakage
• Compatiblewithawiderangeofreducingagents,detergents,denaturants,andotheradditives.
• Reducedsamplepreparationtime,whichminimizesriskofproteindegradationbyproteases.
• Noneedforcentrifugationorfiltrationofthesamples.Directsampleapplication.
• Reliablepurificationofhistidine-taggedproteinsdirectlyfromunclarifiedlysates–simplysonicateandrun.
• Reducedsamplepreparationtime,whichminimizesriskofproteindegradationbyproteases
• Simpleoperationwithasyringe,pump,orchromatographysystemsuchasAKTAdesignorFPLC™System
• Permitrapidyetreliableseparationswithaminimumofsamplepreparationandequipment
Generaldescription
HisTrap™FFcrudecolumnsoffertheconvenienceofsimple,one-steppurificationofhistidine-taggedproteinsdirectlyfromhomogenized,unclarifiedcelllysatebyimmobilizedmetalionaffinitychromatography(IMAC).
HisTrap™FFcrudeisaready-to-usecolumn,prepackedwithprechargedNiSepharose®6FastFlow.Thisprepackedcolumnisintendedforpurificationofhistidine-taggedrecombinantproteinsbyimmobilizedmetalaffinitychromatography(IMAC).Afterthoroughcelldisruption,itispossibletoloadtheunclarifiedlysateonthecolumnwithoutprecentrifugationandfiltrationofthesample.Extendingthedurationofthemechanicaltreatmentofthesampletoensureamorecompletelysisisrecommended.Foroptimalresults,werecommendfirstadditionoflysozymeandDNaseIfollowedbyamechanicallysis,forexamplebysonication.Thehomogenizedsamplecanthenbeloadeddirectlyonthecolumnwithoutaclarificationstepwhichmaypreventdegradationofthetargetproteinandincreasetheactivity.
NiSepharose®6FastFlowhaslownickelion(Ni2+)leakageandiscompatiblewithawiderangeofadditivesusedinproteinpurification.Thespecialdesignofthecolumnincombinationwiththemedium,providefast,simple,andconvenientpurifications.Shortpurificationtimegenerallyminimizesdeleteriouseffects,suchasdegradationandoxidationofsensitivetargetproteins,andisthereforeofgreatimportance.HisTrap™FFcrudecolumnscanbeoperatedwithasyringe,peristalticpump,orliquidchromatographysystemsuchasAKTAdesignchromatographysystems.
LegalInformation
FPLCisatrademarkofGEHealthcare
HisTrapisatrademarkofGEHealthcare
SepharoseisaregisteredtrademarkofGEHealthcare
Safety:
Precautionarystatements
P210-P235-P241-P280-P303+P361+P353-P501
RIDADR
UN1170-class3-PG3-Ethanol,solution