Cyanine3 alkyne is a fluorophore ready for the use in Click Chemistry reaction.
With this reagent, bright and photostable Cyanine3 reporter, an analog of Cy3®, can be attached to nearly any molecule bearing azide group.
This alkyne is non water-soluble. However, when used with organic co-solvent (DMSO or DMF), this dye can be efficiently attached to water-soluble molecules such as proteins, or peptides.
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General properties
Appearance: | red powder |
Molecular weight: | 530.14 |
Molecular formula: | C33H40ClN3O |
Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water |
Quality control: | NMR 1H (95%), HPLC-MS |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
Excitation maximum, nm: | 555 |
ε, L⋅mol−1⋅cm−1: | 150000 |
Emission maximum, nm: | 570 |
Fluorescence quantum yield: | 0.31 |
CF260: | 0.04 |
CF280: | 0.09 |