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Millipore/ECM340 | Human sVCAM-1 ELISA/ECM340/1 kit
  • Millipore/ECM340 | Human sVCAM-1 ELISA/ECM340/1 kit

Millipore/ECM340 | Human sVCAM-1 ELISA/ECM340/1 kit

價格: ￥7560.00 市場價: 12600.00
貨號: ECM340
品牌: Millipore
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    • Description
      CatalogueNumberECM340
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionHumansVCAM-1ELISA
      OverviewVascularcelladhesionmolecule-1(VCAM-1,CD106)isamemberoftheimmunoglobulingenesuperfamily.TwoformsofVCAM-1withsixorsevenextracellularIg-likedomainsariseduetoalternativesplicingfromaseven-domainVCAM-1(7DVCAM-1).7DVCAM-1isthedominantformexpressedbyculturedhumanendothelialcells[Cybulskyetal.,1991;Hessionetal.,1991].Homologyofdomains1through3withdomains4through6suggestsageneduplicationevent[Cybulskyetal.,1991;Hessionetal.,1991].TheCDNAof7DVCAM-1predictsacoreproteinofapproximately81kDawithsevensitesforN-linkedglycosylation.Uponcompleteglycosylationthematureproteinhasamolecularweightofapproximately102kDa.Thisobservationisingeneralagreementwiththe110kDaproteinimmunoprecipitatedfromcytokine-activatedendothelium[Carlosetal.,1990;Riceetal.,1990].

      VCAM-1appearstohavebeenhighlyconservedthroughevolution.BothratandmouseVCAM-1arehighlyhomologousattheproteinleveltothehumanVCAM-1(77%and76%,respectively)[Hessionetal.,1992].

      VCAM-1supportstheadhesionoflymphocytes,monocytes,naturalkillercells,eosinophilsandbasophils,throughitsinteractionwithintegrina4b1(VLA-4).VCAM-1/VLA-4interactionmediatesfirmadherenceofcirculatingnon-neutrophilicleukocytestoendothelium[Shararetal.,1995].VCAM-1alsoparticipatesinleukocyteadhesionoutsideofthevasculature,mediatingprecursorlymphocyteadhesiontobonemarrowstromalcells[Ryanetal.1991]andBcellbindingtolymphnodefolliculardendriticcells[Feedmanetal.,1992].VCAM-1isnotconstitutivelyexpressedonendothelium,butcanbeup-regulatedinvitroinresponsetoLPS,TNF-a,andIL-1[Carlos&Harlan,1990;Osbornetal.,1989],aswellastointerferon-gandIL-4[Masinovskyetal.1990].VCAM-1isalsopresentontissuemacrophages,dendritecells,bonemarrowfibroblasts,myoblastsandmyotubes.

      AsolubleformofVCAM-1hasbeendescribed[DeMaeyer&DeMaeyer-Buignard,1995;Lobbetal.,1991],andsolubleVCAM-1hasbeenfoundinserum[Gearingetal.,1995].IncreasedlevelsofsolubleVCAM-1canbedetectedinseveraldiseasestates,includingcancer[Banksetal.,1993;Fortisetal.,1995;Gearing&Newman,1993;Laietal.,1995],autoimmunedisorders[Gearing&Newman,1993;Doreduffyetal.,1995;Gruschwitzetal.,1995;Masonetal.,1993;Mrowka&Sieberth,1995;Spronketal.,1994;Wellicomeetal.,1993],infections[Jakobsenetal.,1994]andinflammations[Gearing&Newman,1993;Mrowka&Sieberth,1995;Adamsetal.,1995;Limetal.,1995].

      TestPrinciple:

      Ananti-VCAM-1monoclonalantibodyisadsorbedontomicrowells.

      SolubleVCAM-1presentinasampleorstandardthenbindstoantibodiesadsorbedtothemicrowells.Amixtureofbiotin-conjugatedmonoclonalanti-VCAM-1antibodyandStreptavidin-HRPconjugateisadded.Biotinylatedanti-VCAM-1antibodybindstoVCAM-1capturedbythefirstantibody.

      Streptavidin-HRPbindstothebiotinylatedanti-VCAM-1.Unboundbiotinylatedanti-VCAM-1andStreptavidin-HRPconjugateisremovedwithawashstepandHRPsubstratesolutionisaddedtothewells.

      ThepairofmonoclonalantibodiesusedinthisELISAdetectthesolubleformofVCAM-1presentinserum,plasma,urine,andotherBIOLOGicalfluids.

      Anamountofcoloredproductisformed,proportionaltotheamountofsolubleVCAM-1presentinthesample.Thereactionisterminatedbyadditionofacidandabsorbanceismeasuredat450nm.AstandardcurveispreparedfromsixsolubleVCAM-1standarddilutionsandtheVCAM-1sampleconcentrationisdetermined.

      Application:

      TheVCAM-1ELISAisanenzyme-linkedimmunosorbentassayforquantitativedetectionofsolubleVascularCellAdhesionMolecule-1incellculturesupernatants,serum,plasma,orotherbiologicalfluids.TheVCAM-1ELISAisforresearchuseonly.Notforuseindiagnosticprocedures.
      AlternateNames
      • CD106
      MaterialsRequiredbutNotDelivered-5mLand10mLgraduatedPipettes

      -5μLto1000μLadjustablesinglechannelmicropipetteswith

      disposabletips

      -50μLto300μLadjustablemultichannelmicropipettewithdisposabletips

      -Multichannelmicropipettereservoir

      -Beakers,flasks,cylindersnecessaryforpreparationofreagents

      -Devicefordeliveryofwashsolution(multichannelwashbottleor

      automaticwashsystem)

      -MicrowellstripreadercapableofreADIngat450nm(620nmas

      optionalreferencewavelength)

      -Glass-distilledordeionizedwater

      -Statisticalcalculatorwithprogramtoperformlinearregressionanalysis.
      ProductInformation
      Components
      • ·2aluminumpoucheswith3MicrowellStrips(16wellsperstrip)coatedwithMonoclonalAntibody(murine)tohumanVCAM-1
      • ·1vial(0.1mL)ConjugateMixturecontainingBiotin-ConjugatedAnti-VCAM-1Monoclonal(murine)AntibodymixedwithStreptavidin-HRP*
      • ·2vials(200ng/mLasreconstituted)solubleVCAM-1Standard,lyophilized*
      • ·1viallyophilizedcontrolserum
      • ·1bottle(50mL)WashBufferConcentrate20X(PBSwith1%Tween20)*
      • ·1vial(5mL)AssayBufferConcentrate20X(PBSwith1%Tween20and10%BSA)*
      • ·1vial(7mL)SubstrateSolutionI(tetramethyl-benzidine)
      • ·1vial(7mL)SubstrateSolutionII(0.02%bufferedhydrogenperoxide)
      • ·1vial(12mL)StopSolution(1MPhosphoricAcid,H3PO4),readytouse
      • ·1MicrowellStripHolder
      • ·2AdhesivePlateCovers
      • ·reagentscontaining0.01%thimerosalaspreservative
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditionsPrecautions:

      ·Reagentsareintendedforresearchuseonlyandarenotforuseindiagnosticortherapeuticprocedures.

      ·Donotmixorsubstitutereagentswiththosefromotherlotsorothersources.

      ·Donotusekitreagentsbeyondexpirationdateonlabel.

      ·Donotexposekitreagentstostronglightduringstorageorincubation.

      ·Rubberordisposablelatexglovesshouldbewornwhilehandlingkitreagentsorspecimens.

      ·Somereagentscontainthimerosalaspreservative,whichishighlytoxicbyinhalation,ingestion,orcontactwithskin.ThimerosalisapossIBLemutagenandshouldbehandledaccordingly.

      ·Avoidcontactofsubstratesolutionswithoxidizingagentsandmetal.

      ·Inordertoavoidmicrobialcontaminationorcross-contaminationofreagentsorspecimenswhichmayinvalidatethetestusedisposablepipettetipsand/orpipettes.

      ·Useclean,dedicatedreagenttraysfordispensingtheconjugateandsubstratereagents.

      ·Exposuretoacidswillinactivatetheconjugate.

      ·Glass-distilledwaterordeionizedwatermustbeusedforreagent

      preparation.

      ·Substratesolutionsmustbeatroomtemperaturepriortouse.

      ·Sinceexactconditionsmayvaryfromassaytoassay,astandard

      curvemustbeestablishedforeveryrun.

      ·Bacterialorfungalcontaminationofeitherscreensamplesorreagentsorcross-contaminationbetweenreagentsmaycauseerroneousresults.

      ·Disposablepipettetips,flasksorglasswarearepreferred.Reusableglasswaremustbewashedandthoroughlyrinsedofalldetergentsbeforeuse.

      ·Improperorinsufficientwashingatanystageoftheprocedurewillresultineitherfalsepositiveorfalsenegativeresults.CompletelyemptywellsbeforedispensingfreshWashBuffer,fillwithWashBufferasindicatedforeachwashcycleanddonotallowwellstosituncoveredordryforextendedperiods.

      ·Anyhumananti-mouseIgGantibody(HAMA)presentmayinterferewithassaysutilizingmurinemonoclonalantibodiesleadingtobothfalsepositiveandfalsenegativeresults.HAMAinterferencecanbereducedbyaddingmurineimmunoglobulins(serum,asciticfluid,ormonoclonalantibodiesofirrelevantspecificity)totheSampleDiluent.
      Applications
      ApplicationThisHumansVCAM-1ELISAisusedtomeasure&quantifysVCAM-1levelsinInflammation&Immunology&Neuroscienceresearch.
      KeyApplications
      • ELISA
      BiologicalInformation
      SpeciesReactivity
      • Human
      AnalytesAvailable
      • sVCAM-1
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneisamemberoftheIgsuperfamilyandencodesacellsurfacesialoglycoproteinexpressedbycytokine-activatedendothelium.ThistypeImembraneproteinmediatesleukocyte-endothelialcelladhesionandsignaltransduction,andmayplayaroleinthedevelopmentofartherosclerosisandrheumatoidarthritis.Twoalternativelysplicedtranscriptsencodingdifferentisoformshavebeendescribedforthisgene.
      GeneSymbol
      • VCAM1
      • INCAM-100
      • MGC99561
      • CD106
      • DKFZp779G2333
      • L1CAM
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P19320#Importantincell-cellrecognition.Appearstofunctioninleukocyte-endothelialcelladhesion.Interactswiththebeta-1integrinVLA4onleukocytes,andmediatesbothadhesionandsignaltransduction.TheVCAM1/VLA4interactionmayplayapathophysiologicrolebothinimmuneresponsesandinleukocyteemigrationtositesofinflammation.
      SIZE:739aminoacids;81276Da
      SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
      TISSUESPECIFICITY:Expressedoninflamedvascularendothelium,aswellasonmacrophage-likeanddendriticcelltypesinbothnormalandinflamedtissue.
      DOMAIN:SwissProt:P19320EitherthefirstorthefourthIg-likeC2-typedomainisrequiredforVLA4-dependentcelladhesion.
      PTM:Sialoglycoprotein.
      DISEASE:SwissProt:P19320#Mayplayanimportantroleinthegenesisofatherosclerosisandrheumatoidarthritis.
      SIMILARITY:Contains7Ig-likeC2-type(immunoglobulin-like)domains.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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