This protein phosphorylation antibody pair set comes with two antibodies, one against the CDKN1A protein, and the other against the specific T145 phosphorylated site of CDKN1A for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity:
Human
Quality Control Testing:
Dual recognition immunofluorescence result.Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product:
Antibody pair set content: 1. Phospho-CDKN1A T145 rabbit polyclonal antibody (20 ul) With 0.09% sodium azide. 2. CDKN1A mouse monoclonal antibody (40 ug) In 1x PBS, pH 7.2 *Reagents are sufficient for at least 30-50 assays using recommended protocols.
Storage Instruction:
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
Datasheet:
Download
Publication Reference
1.
In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method.Jarvius M, Paulsson J, Weibrecht I, Leuchowius KJ, Andersson AC, Wahlby C, Gullberg M,Botling J,Sjoblom T, Markova B, Ostman A, Landegren U, Soderberg O.Mol Cell Proteomics. 2007 Sep;6(9):1500-9. Epub 2007 Jun 12.
2.
Direct observation of individual endogenous protein complexes in situ by proximity ligation.Soderberg O, Gullberg M, Jarvius M , Ridderstrale K, Leuchowius KJ, Jarvius J, Wester K, Hydbring P, Bahram F, Larsson LG, and Landegren U. Nat Methods. 2006 Dec;3(12):995-1000. Epub 2006 Oct 29.
3.
Cytokine detection by antibody-based proximity ligation.Gullberg M, Gustafsdottir SM, Schallmeiner E, Jarvius J, Bjarnegard M, Betsholtz C, Landegren U, andFredriksson S. Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8420-4. Epub 2004 May 21.
4.
Protein detection using proximity-dependent DNA ligation assays.Fredriksson S, GullbergM, Jarvius J, Olsson C, Pietras K, Gustafsdottir SM, Ostman A, and Landegren U. Nat Biotechnol. 2002 May;20(5):473-7.
5.
Highly specific detection of phosphorylated proteins by DuolinkMats Gullberg and Ann-Catrin Andersson Nature Methods 6. 2009
Applications
In situ Proximity Ligation Assay (Cell)
Application Image
In situ Proximity Ligation Assay (Cell)
Gene Information
Entrez GeneID:
1026
Gene Name:
CDKN1A
Gene Alias:
CAP20,CDKN1,CIP1,MDA-6,P21,SDI1,WAF1,p21CIP1
Gene Description:
cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Omim ID:
116899
Gene Ontology:
Hyperlink
Gene Summary:
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq
Other Designations:
CDK-interaction protein 1,DNA synthesis inhibitor,OTTHUMP00000016298,cyclin-dependent kinase inhibitor 1A,melanoma differentiation associated protein 6,wild-type p53-activated fragment 1
Interactome
Gene Pathway
Bladder cancer
Cell cycle
Chronic myeloid leukemia
ErbB signaling pathway
Glioma
Melanoma
p53 signaling pathway
Pathways in cancer
Prostate cancer
Related Disease
Adenocarcinoma
Ataxia telangiectasia
Atherosclerosis
Atherosclerosis
Bowen"s Disease
Brain Neoplasms
Breast cancer
Breast Neoplasms
Carcinoma
Carcinoma, Basal Cell
Carcinoma, Squamous Cell
Carcinoma, Transitional Cell
Cardiovascular Diseases
Chromosome Aberrations
Chronic Disease
Colorectal Neoplasms
Diabetes Mellitus, Type 2
Disease Progression
DNA Damage
... see more
Edema
Esophageal Neoplasms
Genetic Predisposition to Disease
Glaucoma
Glaucoma, Open-Angle
Glioma
Head and Neck Neoplasms
Helicobacter Infections
Intestinal Neoplasms
Kidney Failure, Chronic
Laryngeal Neoplasms
Leiomyoma
Leukemia, Lymphocytic, Acute, L1
Leukemia, Pre-B-Cell
Low Tension Glaucoma
Lung Neoplasms
Lupus Erythematosus, Systemic
Lupus Nephritis
Lymphoma, Non-Hodgkin
Malignant melanoma
Melanoma
Meningioma
Mouth Neoplasms
Multiple endocrine neoplasia
Multiple Endocrine Neoplasia Type 1
Myocardial Infarction
Nasopharyngeal Neoplasms
Neoplasm Invasiveness
Neoplasm Recurrence, Local
Neoplasms, Glandular and Epithelial
Neoplasms, Second Primary
Neuroma, Acoustic
Occupational Diseases
Ocular Hypertension
Ovarian cancer
Ovarian Neoplasms
Pancreatic cancer
Pancreatic Neoplasms
Papillomavirus Infections
Pharyngeal Neoplasms
Precancerous Conditions
Prostate cancer
Prostatic Hyperplasia
Prostatic Neoplasms
Pulmonary Disease, Chronic Obstructive
Radiation Injuries
Skin Diseases
Skin Neoplasms
Stomach Neoplasms
Urinary Bladder Neoplasms
Uterine Cervical Neoplasms
Uterine Neoplasms
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